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Literature summary for 1.7.2.6 extracted from
- Optimized inhibition assays reveal different inhibitory responses of hydroxylamine oxidoreductases from beta- and gamma-proteobacterial ammonium-oxidizing bacteria (2016), Biochem. Biophys. Res. Commun., 476, 127-133 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no significant changes in the NeHAO and NoHAO activities by addition of 0.5% DMSO and 0.01% Triton X-100 | Nitrosospira multiformis | |
| additional information | no significant changes in the NeHAO and NoHAO activities by addition of 0.5% DMSO and 0.01% Triton X-100 | Nitrosococcus oceani | |
| additional information | no significant changes in the NeHAO and NoHAO activities by addition of 0.5% DMSO and 0.01% Triton X-100 | Nitrosomonas europaea | |
| additional information | no significant changes in the NeHAO and NoHAO activities by addition of 0.5% DMSO and 0.01% Triton X-100 | Nitrosomonas sp. JPCCT2 |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene hao, sequence comparisons | Nitrosospira multiformis |
| gene hao, sequence comparisons | Nitrosococcus oceani |
| gene hao, sequence comparisons | Nitrosomonas europaea |
| gene hao, sequence comparisons | Nitrosomonas sp. JPCCT2 |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| purified enzyme, 2 weeks | Nitrosomonas europaea |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no significant changes in the NeHAO and NoHAO activities by addition of 0.5% DMSO and 0.01% Triton X-100 | Nitrosococcus oceani | |
| additional information | no significant changes in the NeHAO and NoHAO activities by addition of 0.5% DMSO and 0.01% Triton X-100 | Nitrosomonas europaea | |
| additional information | no significant changes in the NeHAO and NoHAO activities by addition of 0.5% DMSO and 0.01% Triton X-100 | Nitrosomonas sp. JPCCT2 | |
| additional information | no significant changes in the NeHAO and NoHAO activities by addition of 0.5% DMSO and 0.01% Triton X-100 | Nitrosospira multiformis | |
| phenylhydrazine | an HAO enzyme specific inhibitor, slight inhibition of enzyme NoHAO activity at 0.01 mM | Nitrosococcus oceani |  |
| phenylhydrazine | an HAO enzyme specific inhibitor, inhibits about 60% of enzyme NeHAO activity at 0.01 mM | Nitrosomonas europaea | |
| phenylhydrazine | an HAO enzyme specific inhibitor | Nitrosomonas sp. JPCCT2 | |
| phenylhydrazine | an HAO enzyme specific inhibitor | Nitrosospira multiformis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | heme | Nitrosospira multiformis | |
| Fe2+ | heme | Nitrosococcus oceani | |
| Fe2+ | heme | Nitrosomonas europaea | |
| Fe2+ | heme | Nitrosomonas sp. JPCCT2 | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hydroxylamine + H2O + 4 ferricytochrome c | Nitrosospira multiformis | - |
nitrite + 4 ferrocytochrome c + 5 H+ | - |
? | |
| hydroxylamine + H2O + 4 ferricytochrome c | Nitrosococcus oceani | - |
nitrite + 4 ferrocytochrome c + 5 H+ | - |
? | |
| hydroxylamine + H2O + 4 ferricytochrome c | Nitrosomonas europaea | - |
nitrite + 4 ferrocytochrome c + 5 H+ | - |
? | |
| hydroxylamine + H2O + 4 ferricytochrome c | Nitrosomonas sp. JPCCT2 | - |
nitrite + 4 ferrocytochrome c + 5 H+ | - |
? | |
| hydroxylamine + H2O + 4 ferricytochrome c | Nitrosomonas europaea ATCC 19718 | - |
nitrite + 4 ferrocytochrome c + 5 H+ | - |
? | |
| hydroxylamine + H2O + 4 ferricytochrome c | Nitrosococcus oceani ATCC 19707 | - |
nitrite + 4 ferrocytochrome c + 5 H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Nitrosococcus oceani | - |
- |
- |
| Nitrosococcus oceani ATCC 19707 | - |
- |
- |
| Nitrosomonas europaea | Q50925 | - |
- |
| Nitrosomonas europaea ATCC 19718 | Q50925 | - |
- |
| Nitrosomonas sp. JPCCT2 | - |
- |
- |
| Nitrosospira multiformis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme from strain ATCC 19718 by anion exchange chromatography and gel filtration | Nitrosococcus oceani |
| native enzyme from strain ATCC 19718 by anion exchange chromatography, gel filtration, and hdroxyapatite chromatography | Nitrosomonas europaea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hydroxylamine + H2O + 4 ferricytochrome c | - |
Nitrosospira multiformis | nitrite + 4 ferrocytochrome c + 5 H+ | - |
? | |
| hydroxylamine + H2O + 4 ferricytochrome c | - |
Nitrosococcus oceani | nitrite + 4 ferrocytochrome c + 5 H+ | - |
? | |
| hydroxylamine + H2O + 4 ferricytochrome c | - |
Nitrosomonas europaea | nitrite + 4 ferrocytochrome c + 5 H+ | - |
? | |
| hydroxylamine + H2O + 4 ferricytochrome c | - |
Nitrosomonas sp. JPCCT2 | nitrite + 4 ferrocytochrome c + 5 H+ | - |
? | |
| hydroxylamine + H2O + 4 ferricytochrome c | usage of horse heart cytochrome c | Nitrosospira multiformis | nitrite + 4 ferrocytochrome c + 5 H+ | - |
? | |
| hydroxylamine + H2O + 4 ferricytochrome c | usage of horse heart cytochrome c | Nitrosococcus oceani | nitrite + 4 ferrocytochrome c + 5 H+ | - |
? | |
| hydroxylamine + H2O + 4 ferricytochrome c | usage of horse heart cytochrome c | Nitrosomonas europaea | nitrite + 4 ferrocytochrome c + 5 H+ | - |
? | |
| hydroxylamine + H2O + 4 ferricytochrome c | usage of horse heart cytochrome c | Nitrosomonas sp. JPCCT2 | nitrite + 4 ferrocytochrome c + 5 H+ | - |
? | |
| hydroxylamine + H2O + 4 ferricytochrome c | - |
Nitrosomonas europaea ATCC 19718 | nitrite + 4 ferrocytochrome c + 5 H+ | - |
? | |
| hydroxylamine + H2O + 4 ferricytochrome c | usage of horse heart cytochrome c | Nitrosomonas europaea ATCC 19718 | nitrite + 4 ferrocytochrome c + 5 H+ | - |
? | |
| hydroxylamine + H2O + 4 ferricytochrome c | - |
Nitrosococcus oceani ATCC 19707 | nitrite + 4 ferrocytochrome c + 5 H+ | - |
? | |
| hydroxylamine + H2O + 4 ferricytochrome c | usage of horse heart cytochrome c | Nitrosococcus oceani ATCC 19707 | nitrite + 4 ferrocytochrome c + 5 H+ | - |
? | |
| additional information | optimization of HAO activity assay method, evaluation, overview | Nitrosospira multiformis | ? | - |
? | |
| additional information | optimization of HAO activity assay method, evaluation, overview | Nitrosococcus oceani | ? | - |
? | |
| additional information | optimization of HAO activity assay method, evaluation, overview | Nitrosomonas europaea | ? | - |
? | |
| additional information | optimization of HAO activity assay method, evaluation, overview | Nitrosomonas sp. JPCCT2 | ? | - |
? | |
| additional information | optimization of HAO activity assay method, evaluation, overview | Nitrosomonas europaea ATCC 19718 | ? | - |
? | |
| additional information | optimization of HAO activity assay method, evaluation, overview | Nitrosococcus oceani ATCC 19707 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HAO | - |
Nitrosospira multiformis |
| HAO | - |
Nitrosococcus oceani |
| HAO | - |
Nitrosomonas europaea |
| HAO | - |
Nitrosomonas sp. JPCCT2 |
| hydroxylamine oxidoreductase | - |
Nitrosospira multiformis |
| hydroxylamine oxidoreductase | - |
Nitrosococcus oceani |
| hydroxylamine oxidoreductase | - |
Nitrosomonas europaea |
| hydroxylamine oxidoreductase | - |
Nitrosomonas sp. JPCCT2 |
| NeHAO | - |
Nitrosomonas europaea |
| NoHAO | - |
Nitrosococcus oceani |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Nitrosospira multiformis |
| 25 | - |
assay at | Nitrosococcus oceani |
| 25 | - |
assay at | Nitrosomonas europaea |
| 25 | - |
assay at | Nitrosomonas sp. JPCCT2 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Nitrosospira multiformis |
| 7 | - |
assay at | Nitrosomonas sp. JPCCT2 |
| 9.4 | - |
- |
Nitrosomonas europaea |
| 10 | - |
- |
Nitrosococcus oceani |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome c | with heme | Nitrosospira multiformis | |
| cytochrome c | with heme | Nitrosococcus oceani | |
| cytochrome c | with heme | Nitrosomonas europaea | |
| cytochrome c | with heme | Nitrosomonas sp. JPCCT2 | |
| heme | c-type hemes and P-460-type heme | Nitrosospira multiformis | |
| heme | c-type hemes and P-460-type heme | Nitrosococcus oceani | |
| heme | c-type hemes and P-460-type heme | Nitrosomonas europaea | |
| heme | c-type hemes and P-460-type heme | Nitrosomonas sp. JPCCT2 | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.00092 | - |
pH 7.0, 25°C | Nitrosomonas sp. JPCCT2 | phenylhydrazine | |
| 0.00101 | - |
pH 7.0, 25°C | Nitrosomonas europaea | phenylhydrazine | |
| 0.00123 | - |
pH 7.0, 25°C | Nitrosospira multiformis | phenylhydrazine | |
| 0.00289 | - |
pH 7.0, 25°C | Nitrosococcus oceani | phenylhydrazine | |
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