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Literature summary for 1.7.2.5 extracted from

  • Matsuda, Y.; Inamori, K.; Osaki, T.; Eguchi, A.; Watanabe, A.; Kawabata, S.; Iba, K.; Arata, H.
    Nitric oxide-reductase homologue that contains a copper atom and has cytochrome c-oxidase activity from an aerobic phototrophic bacterium Roseobacter denitrificans (2002), J. Biochem., 131, 791-800.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
additional information oxidation of NADH by membranes is inhibited almost completely by antimycin A or myxothiazole, indicating that the bacterium does not have a quinol oxidase Roseobacter denitrificans

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ protein contains heme c, heme b, and copper in a 1:2:1 stoichiometry Roseobacter denitrificans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
17600
-
calculated, small subunit Roseobacter denitrificans
18000
-
composed of two subunits with molecular weights on SDS-PAGE of 1 * 37000 and 1 * 18000 Roseobacter denitrificans
37000
-
composed of two subunits with molecular weights on SDS-PAGE of 1 * 37000 and 1 * 18000 Roseobacter denitrificans
49000
-
calculated, large subunit Roseobacter denitrificans

Organism

Organism UniProt Comment Textmining
Roseobacter denitrificans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sepharose CL-6B anion-exchange chromatography, Sephacryl S-300 gel filtration, and Mono Q anion-exchange chromatography. Enzyme is solubilized with sucrose monodecanoate from the membranes of Roseobacter denitrificans grown aerobically under light conditions, and purified to electrophoretic homogeneity Roseobacter denitrificans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.3
-
last purification step Roseobacter denitrificans

Subunits

Subunits Comment Organism
dimer composed of two subunits with molecular weights on SDS-PAGE of 1 * 37000 and 1 * 18000 Roseobacter denitrificans

Synonyms

Synonyms Comment Organism
nitric oxide-reductase NorBC homologue, cytochrome cb-type enzyme with cytochrome c-oxidase activity Roseobacter denitrificans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Roseobacter denitrificans

Cofactor

Cofactor Comment Organism Structure
heme protein contains heme c, heme b, and copper in a 1:2:1 stoichiometry Roseobacter denitrificans