Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | oxidation of NADH by membranes is inhibited almost completely by antimycin A or myxothiazole, indicating that the bacterium does not have a quinol oxidase | Roseobacter denitrificans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | protein contains heme c, heme b, and copper in a 1:2:1 stoichiometry | Roseobacter denitrificans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
17600 | - |
calculated, small subunit | Roseobacter denitrificans |
18000 | - |
composed of two subunits with molecular weights on SDS-PAGE of 1 * 37000 and 1 * 18000 | Roseobacter denitrificans |
37000 | - |
composed of two subunits with molecular weights on SDS-PAGE of 1 * 37000 and 1 * 18000 | Roseobacter denitrificans |
49000 | - |
calculated, large subunit | Roseobacter denitrificans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Roseobacter denitrificans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-Sepharose CL-6B anion-exchange chromatography, Sephacryl S-300 gel filtration, and Mono Q anion-exchange chromatography. Enzyme is solubilized with sucrose monodecanoate from the membranes of Roseobacter denitrificans grown aerobically under light conditions, and purified to electrophoretic homogeneity | Roseobacter denitrificans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.3 | - |
last purification step | Roseobacter denitrificans |
Subunits | Comment | Organism |
---|---|---|
dimer | composed of two subunits with molecular weights on SDS-PAGE of 1 * 37000 and 1 * 18000 | Roseobacter denitrificans |
Synonyms | Comment | Organism |
---|---|---|
nitric oxide-reductase | NorBC homologue, cytochrome cb-type enzyme with cytochrome c-oxidase activity | Roseobacter denitrificans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Roseobacter denitrificans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | protein contains heme c, heme b, and copper in a 1:2:1 stoichiometry | Roseobacter denitrificans |