KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics, overview | Achromobacter cycloclastes |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | a copper enzyme, conformational changes of the CuA center may affect electron transfer from the physiological electron donor and the electron-transfer rate from CuA to CuZ, overview | Achromobacter cycloclastes |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrogen + H2O + acceptor | Achromobacter cycloclastes | - |
nitrous oxide + reduced acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Achromobacter cycloclastes | P94127 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
145.6 | - |
- |
Achromobacter cycloclastes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrogen + H2O + acceptor | - |
Achromobacter cycloclastes | nitrous oxide + reduced acceptor | - |
? | |
nitrogen + H2O + acceptor | under some conditions, electron transfer may be rate limiting in N2O reduction. The transition state is stabilized by H-bonding interactions between the active site and an N2O-derived intermediate bound to the catalytic CuZ cluster | Achromobacter cycloclastes | nitrous oxide + reduced acceptor | - |
? |
Synonyms | Comment | Organism |
---|---|---|
N2O reductase | - |
Achromobacter cycloclastes |
N2OR | - |
Achromobacter cycloclastes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.9 | - |
- |
Achromobacter cycloclastes |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.7 | 9.4 | pH profile with two maxima, high complexity of pH dependence, overview | Achromobacter cycloclastes |