General Stability | Organism |
---|---|
an enzyme concentration of at least 0.01 mg per ml is necessary to avoid loss of activity due to dilution | Paracoccus pantotrophus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Acetylene | - |
Paracoccus pantotrophus | |
KCN | - |
Paracoccus pantotrophus | |
NaN3 | - |
Paracoccus pantotrophus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu | wild-type 7.2 atoms per protein dimer | Paracoccus pantotrophus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66281 | - |
2 * 66281, ESI mass spectrometry | Paracoccus pantotrophus |
73000 | - |
2 * 73000, SDS-PAGE | Paracoccus pantotrophus |
115000 | - |
gel filtration | Paracoccus pantotrophus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrous oxide + reduced acceptor | Paracoccus pantotrophus | - |
nitrogen + H2O + acceptor | - |
? | |
nitrous oxide + reduced acceptor | Paracoccus pantotrophus LMD 82.5 | - |
nitrogen + H2O + acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paracoccus pantotrophus | - |
LMD 82.5 | - |
Paracoccus pantotrophus | - |
formerly Thiosphaera pantotropha | - |
Paracoccus pantotrophus LMD 82.5 | - |
LMD 82.5 | - |
Purification (Comment) | Organism |
---|---|
of anaerobically grown cells | Paracoccus pantotrophus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1 | - |
ascorbate-reduced phenanzine methosulphate electrode assay | Paracoccus pantotrophus |
2.6 | - |
- |
Paracoccus pantotrophus |
3.3 | - |
photoreduced methyl viologen spectrophotometric assay | Paracoccus pantotrophus |
3.7 | - |
dithionite-reduced methyl viologen electrode assay | Paracoccus pantotrophus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrous oxide + reduced acceptor | - |
Paracoccus pantotrophus | nitrogen + H2O + acceptor | - |
? | |
nitrous oxide + reduced acceptor | dithionite-reduced methyl viologen as electron donor | Paracoccus pantotrophus | nitrogen + H2O + acceptor | - |
? | |
nitrous oxide + reduced acceptor | - |
Paracoccus pantotrophus LMD 82.5 | nitrogen + H2O + acceptor | - |
? | |
nitrous oxide + reduced acceptor | dithionite-reduced methyl viologen as electron donor | Paracoccus pantotrophus LMD 82.5 | nitrogen + H2O + acceptor | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 73000, SDS-PAGE | Paracoccus pantotrophus |
homodimer | 2 * 66281, ESI mass spectrometry | Paracoccus pantotrophus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
above pH 8 with reduced methyl viologen | Paracoccus pantotrophus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000025 | - |
KCN | - |
Paracoccus pantotrophus | |
0.00064 | - |
NaN3 | - |
Paracoccus pantotrophus | |
0.035 | - |
Acetylene | - |
Paracoccus pantotrophus |