Cloned (Comment) | Organism |
---|---|
gene torD, DNA sequence determination and analysis, overexpression in Escherichia coli strain BL21(DE3) as His-tagged protein | Shewanella massilia |
Crystallization (Comment) | Organism |
---|---|
recombinant monomeric, dimeric, and trimeric forms, hanging drop vapour diffusion method, 4°C, 0.001 ml of 1.2 mg/ml protein in 20 mM Tris-HCl, pH 8.0, 220 mM NaCl, 10 mM DTT, mixed with equal volume of reservoir solution containing 1.6 M ammonium sulfate, 100 mM MES, pH 6.4, 4-6 days, cryoprotection by addition of 15% w/v ethylene glycerol to the reservoir solution, X-ray diffraction structure determination and analysis at 2.42 A resolution | Shewanella massilia |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Shewanella massilia | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
19500 | - |
1 * 24100, monomeric form, small-angle X-ray scattering, 1 * 19500, monomeric form, Guinier analysis, 1 * 25000, monomeric form, non-denaturing PAGE, 1 * 24354-24356, mass spectrometry and sedimentation equilibrium analysis | Shewanella massilia |
24100 | - |
monomeric form, small-angle X-ray scattering | Shewanella massilia |
24350 | 24360 | monomeric form, mass spectrometry and sedimentation equilibrium analysis | Shewanella massilia |
24356 | - |
2 * 24356, sedimentation equilibrium analysis, 2 * 27900, dimeric form, Guinier analysis | Shewanella massilia |
25000 | - |
1 * 24100, monomeric form, small-angle X-ray scattering, 1 * 19500, monomeric form, Guinier analysis, 1 * 25000, monomeric form, non-denaturing PAGE, 1 * 24354-24356, mass spectrometry and sedimentation equilibrium analysis | Shewanella massilia |
27900 | - |
2 * 24356, sedimentation equilibrium analysis, 2 * 27900, dimeric form, Guinier analysis | Shewanella massilia |
47800 | - |
dimeric form, small-angle X-ray scattering | Shewanella massilia |
48710 | - |
dimeric form, mass spectrometry and sedimentation equilibrium analysis | Shewanella massilia |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Shewanella massilia | enzyme is probably required for acquisition of molybdenum cofactor and translocation of the trimethylamine reductase TorA, EC 1.6.6.9, monomeric and dimeric enzyme forms bind to Tor A, the dimeric form binds more efficiently | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Shewanella massilia | - |
TorD, cytoplasmic chaperone of trimethylamine reductase TorA, EC 1.6.6.9, encoded in the tor operon together with TorA and a pentahemic c-type cytochrome | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged monomeric, dimeric, and trimeric forms from Escherichia coli strain BL21(DE3) to homogeneity by nickel affinity chromatography, and gel filtration for the the monomeric form | Shewanella massilia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme is probably required for acquisition of molybdenum cofactor and translocation of the trimethylamine reductase TorA, EC 1.6.6.9, monomeric and dimeric enzyme forms bind to Tor A, the dimeric form binds more efficiently | Shewanella massilia | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 24356, sedimentation equilibrium analysis, 2 * 27900, dimeric form, Guinier analysis | Shewanella massilia |
monomer | 1 * 24100, monomeric form, small-angle X-ray scattering, 1 * 19500, monomeric form, Guinier analysis, 1 * 25000, monomeric form, non-denaturing PAGE, 1 * 24354-24356, mass spectrometry and sedimentation equilibrium analysis | Shewanella massilia |
More | enzyme forms multiple and stable oligomeric species, e.g. monomers, dimers, and trimers, analysis by ultracentrifugation, small-angle X-ray scattering, preliminary diffraction, and circular dichroism spectra, interconversion of the native oligomeric forms at pH 3.0 | Shewanella massilia |
Synonyms | Comment | Organism |
---|---|---|
TorD | - |
Shewanella massilia |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome c | pentahemic | Shewanella massilia |