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Literature summary for 1.7.2.3 extracted from
- Involvement of a mate chaperone (TorD) in the maturation pathway of molybdoenzyme TorA (2003), J. Biol. Chem., 278, 28787-28792.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in strain BL21(DE3) as His-tagged protein | Escherichia coli |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 22500 | - |
x * 22500, recombinant enzyme, SDS-PAGE | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Escherichia coli | TorD chaperone is a chaperone of trimethylamine oxide reductase, EC 1.6.6.9, addition of the chaperone activates the TorA apoenzyme up to 4fold, allowing its maturation, in absence or presence of the TorA molybdenum cofactor, TorD modifies the TorA apoenzyme conformation in absence of the cofactor, probably making the apoenzyme competent for cofactor binding, binding study | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
TorD is a chaperone of TorA, EC 1.6.6.9 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from strain BL21(DE3) to near homogeneity | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | TorD chaperone is a chaperone of trimethylamine oxide reductase, EC 1.6.6.9, addition of the chaperone activates the TorA apoenzyme up to 4fold, allowing its maturation, in absence or presence of the TorA molybdenum cofactor, TorD modifies the TorA apoenzyme conformation in absence of the cofactor, probably making the apoenzyme competent for cofactor binding, binding study | Escherichia coli | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 22500, recombinant enzyme, SDS-PAGE | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TorD | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Escherichia coli |
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