Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Escherichia coli |
overexpression | Shewanella massilia |
overexpression as His-tagged protein | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Escherichia coli | 16020 | - |
soluble | - |
Escherichia coli | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe-S cluster | - |
Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Escherichia coli | the enzyme binds to the trimethylamine oxide reductase TorA apoenzyme, EC 1.6.6.9, recognizing a signal peptide, and allows TorA to bind the essential molybdenum cofactor for transport from the periplasm across the cytoplasmic membrane, TorD is not involved in the transport itself, TorD has a regulatory and controlling function on TorA assembly | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Shewanella massilia | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HiTrap Q FF column chromatography | Escherichia coli |
recombinant His-tagged enzyme by nickel affinity and anion exchange chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme binds to the trimethylamine oxide reductase TorA apoenzyme, EC 1.6.6.9, recognizing a signal peptide, and allows TorA to bind the essential molybdenum cofactor for transport from the periplasm across the cytoplasmic membrane, TorD is not involved in the transport itself, TorD has a regulatory and controlling function on TorA assembly | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Escherichia coli |
More | overexpressed enzyme forms monomers and dimers, a subunit contains 2 distinct domains separated by a short hinge region, dimerization by domain-swapping | Shewanella massilia |
trimer | - |
Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
More | enzyme belongs to the TorD-family chaperones | Escherichia coli |
More | enzyme belongs to the TorD-family chaperones | Shewanella massilia |
TMAO reductase | - |
Escherichia coli |
TorD | - |
Escherichia coli |
TorD | - |
Shewanella massilia |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
molybdopterin guanine dinucleotide | - |
Escherichia coli |