Cloned (Comment) | Organism |
---|---|
amplification of the structural torA gene encoding the enzyme and iits flanking regions using PCR techniques, the torA gene is the third gene of a TMAO-inducible operon, torECAD, encoding the TMAO respiratory components, the torC gene encodes a pentahemic c-type cytochrome, likely to be involved in electron transfer to the TorA terminal reductase, the role of the torE gene is unknown, torD gene encodes the TorD protein, a cytoplasmic protein involved in the folding process of the TorA precursor protein | Shewanella massilia |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
Trimethylamine N-oxide | - |
Shewanella massilia | |
0.022 | - |
4-methylmorpholine N-oxide | - |
Shewanella massilia | |
0.61 | - |
alpha-picoline N-oxide | - |
Shewanella massilia | |
1 | - |
N,N-dimethyldodecylamine N-oxide | - |
Shewanella massilia |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | - |
Shewanella massilia | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Molybdenum | - |
Shewanella massilia |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
90000 | - |
1 * 90000, SDS-PAGE | Shewanella massilia |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
trimethylamine-N-oxide + electron donor | Shewanella massilia | - |
trimethylamine + oxidized electron donor + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Shewanella massilia | O87948 | - |
- |
Purification (Comment) | Organism |
---|---|
using ion exchange chromatography on DE52 column, chromatography on a Mono Q HR 16/10 column and preparative electrophoresis | Shewanella massilia |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Shewanella massilia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylmorpholine N-oxide + electron donor | - |
Shewanella massilia | 4-methylmorpholine + oxidized electron donor + H2O | - |
? | |
alpha-picoline N-oxide + electron donor | alpha-picoline N-oxide is not an efficient substrate for the enzyme | Shewanella massilia | alpha-picoline + H2O + oxidized electron donor | - |
? | |
dimethylsulfoxide + electron donor | - |
Shewanella massilia | ? + oxidized electron donor + H2O | - |
? | |
diphenylsulfoxide + electron donor | - |
Shewanella massilia | diphenylsulfide + oxidized electron donor + H2O | - |
? | |
L-methionine sulfoxide + electron donor | - |
Shewanella massilia | L-methionine + oxidized electron donor + H2O | - |
? | |
N,N-dimethyldodecylamine N-oxide + electron donor | N,N-dimethyldodecylamine N-oxide is not an efficient substrate for the enzyme | Shewanella massilia | N,N-dimethyldodecylamine + H2O + oxidized electron donor | - |
? | |
nitrite + dibutylsulfoxide | - |
Shewanella massilia | ? | - |
? | |
nitrite + DL-methionine sulfoxide | - |
Shewanella massilia | ? | - |
? | |
trimethylamine N-oxide + electron donor | - |
Shewanella massilia | trimethylamine + oxidized electron donor + H2O | - |
? | |
trimethylamine-N-oxide + electron donor | - |
Shewanella massilia | trimethylamine + oxidized electron donor + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 90000, SDS-PAGE | Shewanella massilia |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4 | - |
Dimethylsulfoxide | value below | Shewanella massilia | |
4 | - |
diphenylsulfoxide | value below | Shewanella massilia | |
4 | - |
dibutylsulfoxide | value below | Shewanella massilia | |
4 | - |
DL-methionine sulfoxide | value below | Shewanella massilia | |
78 | - |
alpha-picoline N-oxide | - |
Shewanella massilia | |
109 | - |
4-methylmorpholine N-oxide | - |
Shewanella massilia | |
120 | - |
Trimethylamine N-oxide | - |
Shewanella massilia | |
151 | - |
N,N-dimethyldodecylamine N-oxide | - |
Shewanella massilia |