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Literature summary for 1.7.2.3 extracted from

  • Dos Santos, J.; Iobbi-Nivol, C.; Couillault, C; Giordano, G.; Mejean, V.
    Molecular analysis of the trimethylamine N-oxide (TMAO) reductase respiratory system from a Shewanella species (1998), J. Mol. Biol., 284, 421-433.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
amplification of the structural torA gene encoding the enzyme and iits flanking regions using PCR techniques, the torA gene is the third gene of a TMAO-inducible operon, torECAD, encoding the TMAO respiratory components, the torC gene encodes a pentahemic c-type cytochrome, likely to be involved in electron transfer to the TorA terminal reductase, the role of the torE gene is unknown, torD gene encodes the TorD protein, a cytoplasmic protein involved in the folding process of the TorA precursor protein Shewanella massilia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.017
-
Trimethylamine N-oxide
-
Shewanella massilia
0.022
-
4-methylmorpholine N-oxide
-
Shewanella massilia
0.61
-
alpha-picoline N-oxide
-
Shewanella massilia
1
-
N,N-dimethyldodecylamine N-oxide
-
Shewanella massilia

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
Shewanella massilia
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Molybdenum
-
Shewanella massilia

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
90000
-
1 * 90000, SDS-PAGE Shewanella massilia

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
trimethylamine-N-oxide + electron donor Shewanella massilia
-
trimethylamine + oxidized electron donor + H2O
-
?

Organism

Organism UniProt Comment Textmining
Shewanella massilia O87948
-
-

Purification (Commentary)

Purification (Comment) Organism
using ion exchange chromatography on DE52 column, chromatography on a Mono Q HR 16/10 column and preparative electrophoresis Shewanella massilia

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Shewanella massilia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylmorpholine N-oxide + electron donor
-
Shewanella massilia 4-methylmorpholine + oxidized electron donor + H2O
-
?
alpha-picoline N-oxide + electron donor alpha-picoline N-oxide is not an efficient substrate for the enzyme Shewanella massilia alpha-picoline + H2O + oxidized electron donor
-
?
dimethylsulfoxide + electron donor
-
Shewanella massilia ? + oxidized electron donor + H2O
-
?
diphenylsulfoxide + electron donor
-
Shewanella massilia diphenylsulfide + oxidized electron donor + H2O
-
?
L-methionine sulfoxide + electron donor
-
Shewanella massilia L-methionine + oxidized electron donor + H2O
-
?
N,N-dimethyldodecylamine N-oxide + electron donor N,N-dimethyldodecylamine N-oxide is not an efficient substrate for the enzyme Shewanella massilia N,N-dimethyldodecylamine + H2O + oxidized electron donor
-
?
nitrite + dibutylsulfoxide
-
Shewanella massilia ?
-
?
nitrite + DL-methionine sulfoxide
-
Shewanella massilia ?
-
?
trimethylamine N-oxide + electron donor
-
Shewanella massilia trimethylamine + oxidized electron donor + H2O
-
?
trimethylamine-N-oxide + electron donor
-
Shewanella massilia trimethylamine + oxidized electron donor + H2O
-
?

Subunits

Subunits Comment Organism
monomer 1 * 90000, SDS-PAGE Shewanella massilia

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4
-
Dimethylsulfoxide value below Shewanella massilia
4
-
diphenylsulfoxide value below Shewanella massilia
4
-
dibutylsulfoxide value below Shewanella massilia
4
-
DL-methionine sulfoxide value below Shewanella massilia
78
-
alpha-picoline N-oxide
-
Shewanella massilia
109
-
4-methylmorpholine N-oxide
-
Shewanella massilia
120
-
Trimethylamine N-oxide
-
Shewanella massilia
151
-
N,N-dimethyldodecylamine N-oxide
-
Shewanella massilia