Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Trypanosoma brucei |
Crystallization (Comment) | Organism |
---|---|
mutant C318A, sitting drop vapor diffusion method, using 0.1 M imidazole-HCl (pH 8.0), 3% (w/v) PEG 3350, and 0.35 M Li2SO4 | Trypanosoma brucei |
Protein Variants | Comment | Organism |
---|---|---|
C318A | inactive | Trypanosoma brucei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ribavirin 5'-monophosphate | - |
Trypanosoma brucei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
494000 | - |
gel filtration | Trypanosoma brucei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GMP + NADPH + H+ | Trypanosoma brucei | - |
IMP + NH3 + NADP+ | - |
? | |
GMP + NADPH + H+ | Trypanosoma brucei ILTat | - |
IMP + NH3 + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei | - |
- |
- |
Trypanosoma brucei ILTat | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni Sepharose 6 column chromatography and Sephacryl S-200HR gel filtration | Trypanosoma brucei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GMP + NADPH + H+ | - |
Trypanosoma brucei | IMP + NH3 + NADP+ | - |
? | |
GMP + NADPH + H+ | - |
Trypanosoma brucei ILTat | IMP + NH3 + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homooctamer | - |
Trypanosoma brucei |
Synonyms | Comment | Organism |
---|---|---|
GMPR | - |
Trypanosoma brucei |
guanosine 5'-monophosphate reductase | - |
Trypanosoma brucei |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Trypanosoma brucei |