Any feedback?
Literature summary for 1.7.1.7 extracted from
- NADPH-dependent GMP reductase isoenzyme of human (GMPR2). Expression, purification, and kinetic properties (2002), Int. J. Biochem. Cell Biol., 34, 1035-1050.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no sulfhydryl donor required for activity | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 1 mM, 49% inhibition | Homo sapiens |  |
| Cu2+ | 1 mM, more than 90% inhibition | Homo sapiens | |
| GTP | 0.05 mM, 30% inhibition | Homo sapiens | |
| Mg2+ | 1 mM, 9% inhibition | Homo sapiens | |
| additional information | not inhibited by ATP | Homo sapiens | |
| Ni2+ | 1 mM, more than 90% inhibition | Homo sapiens | |
| XMP | 0.05 mM, 99% inhibition | Homo sapiens | |
| XTP | 0.05 mM, 50% inhibition | Homo sapiens | |
| Zn2+ | 1 mM, 86% inhibition | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0174 | - |
GMP | - |
Homo sapiens | |
| 0.0266 | - |
NADPH | - |
Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
x * 40000, recombinant His-tagged enzyme, SDS-PAGE | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Homo sapiens | Q9P2T1 | - |
- |
| no activity in Haemophilus influenzae | - |
- |
- |
| no activity in Methanocaldococcus jannaschii | - |
other name Methanococcus jannaschii | - |
| no activity in Mycoplasma genitalium | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | strong expression | Homo sapiens | - |
| cardiac muscle | - |
Homo sapiens | - |
| colon | expressed at low levels | Homo sapiens | - |
| kidney | - |
Homo sapiens | - |
| leukocyte | expressed at low levels | Homo sapiens | - |
| liver | strong expression | Homo sapiens | - |
| placenta | strong expression | Homo sapiens | - |
| skeletal muscle | - |
Homo sapiens | - |
| thymus | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| NADPH + guanosine 5'-phosphate | - |
Homo sapiens | NADP+ + inosine 5'-phosphate + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 40000, recombinant His-tagged enzyme, SDS-PAGE | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.1 | - |
enzyme shows 2 maxima | Homo sapiens |
| 7.8 | - |
enzyme shows 2 maxima, strong decrease above | Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8.2 | strong decrease above | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Homo sapiens | |
| NADPH | no activity with NADH | Homo sapiens | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
