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Literature summary for 1.7.1.6 extracted from

  • Ryan, A.; Wang, C.; Laurieri, N.; Westwood, I.; Sim, E.
    Reaction mechanism of azoreductases suggests convergent evolution with quinone oxidoreductases (2010), Protein Cell, 1, 780-790.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
Methyl red substrate inhibition above 0.08 mM Pseudomonas aeruginosa
NaCl more than 40% of maximum activity up to 2.5 M Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.055
-
balsalzide isoform AzoR2, pH 8.0, temperature not specified in the publication Pseudomonas aeruginosa
0.22
-
sulfasalazine isoform AzoR3, pH 8.0, temperature not specified in the publication Pseudomonas aeruginosa
0.26
-
Methyl red isoform AzoR1, pH 8.0, temperature not specified in the publication Pseudomonas aeruginosa
0.298
-
NADH isoform AzoR1, pH 8.0, temperature not specified in the publication Pseudomonas aeruginosa
0.3
-
Methyl red isoform AzoR2, pH 8.0, temperature not specified in the publication Pseudomonas aeruginosa
0.34
-
balsalzide isoform AzoR3, pH 8.0, temperature not specified in the publication Pseudomonas aeruginosa
0.38
-
sulfasalazine isoform AzoR2, pH 8.0, temperature not specified in the publication Pseudomonas aeruginosa
0.525
-
NADH isoform AzoR1, pH 8.0, temperature not specified in the publication Pseudomonas aeruginosa
0.983
-
Methyl red isoform AzoR3, pH 8.0, temperature not specified in the publication Pseudomonas aeruginosa
1.118
-
sulfasalazine isoform AzoR1, pH 8.0, temperature not specified in the publication Pseudomonas aeruginosa
2.447
-
balsalzide isoform AzoR1, pH 8.0, temperature not specified in the publication Pseudomonas aeruginosa
10.26
-
NADPH isoform AzoR1, pH 8.0, temperature not specified in the publication Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-

Reaction

Reaction Comment Organism Reaction ID
N,N-dimethyl-1,4-phenylenediamine + aniline + 2 NADP+ = 4-(dimethylamino)azobenzene + 2 NADPH + 2 H+ ping-pong mechanism Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
balsalzide + NADH + H+
-
Pseudomonas aeruginosa ? + NAD+
-
?
methyl red + NADPH + H+
-
Pseudomonas aeruginosa ? + NADP+
-
?
additional information no substrate: azobenzene Pseudomonas aeruginosa ?
-
?
sulfasalazine + NADPH + H+
-
Pseudomonas aeruginosa ? + NADP+
-
?

Synonyms

Synonyms Comment Organism
AzoR1
-
Pseudomonas aeruginosa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.2
-
balsalzide isoform AzoR3, pH 8.0, temperature not specified in the publication Pseudomonas aeruginosa
0.5
-
sulfasalazine isoform AzoR3, pH 8.0, temperature not specified in the publication Pseudomonas aeruginosa
0.7
-
balsalzide isoform AzoR2, pH 8.0, temperature not specified in the publication Pseudomonas aeruginosa
0.8
-
sulfasalazine isoform AzoR2, pH 8.0, temperature not specified in the publication Pseudomonas aeruginosa
1
-
Methyl red isoform AzoR2, pH 8.0, temperature not specified in the publication Pseudomonas aeruginosa
2.6
-
sulfasalazine isoform AzoR1, pH 8.0, temperature not specified in the publication Pseudomonas aeruginosa
4.8
-
Methyl red isoform AzoR1, pH 8.0, temperature not specified in the publication Pseudomonas aeruginosa
11.6
-
Methyl red isoform AzoR3, pH 8.0, temperature not specified in the publication Pseudomonas aeruginosa
20
-
balsalzide isoform AzoR1, pH 8.0, temperature not specified in the publication Pseudomonas aeruginosa

Cofactor

Cofactor Comment Organism Structure
FMN
-
Pseudomonas aeruginosa
NADH isoforms AzoR2 and AzoR3 prefer NADH over NADPH Pseudomonas aeruginosa
NADPH isoform AzoR1 prefers NADPH over NADH Pseudomonas aeruginosa

Expression

Organism Comment Expression
Pseudomonas aeruginosa in presence of methyl red, expression of isoform AzoR1 increases more than 50% down
Pseudomonas aeruginosa in presence of balsalazide, expression of isoform AzoR1 increases by about 50% up
Pseudomonas aeruginosa in presence of methyl red or balsalzide, expression of isoform AzoR2 increases 3.5- and 2.5fold, respectively up