Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Methyl red | substrate inhibition above 0.08 mM | Pseudomonas aeruginosa | |
NaCl | more than 40% of maximum activity up to 2.5 M | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.055 | - |
balsalzide | isoform AzoR2, pH 8.0, temperature not specified in the publication | Pseudomonas aeruginosa | |
0.22 | - |
sulfasalazine | isoform AzoR3, pH 8.0, temperature not specified in the publication | Pseudomonas aeruginosa | |
0.26 | - |
Methyl red | isoform AzoR1, pH 8.0, temperature not specified in the publication | Pseudomonas aeruginosa | |
0.298 | - |
NADH | isoform AzoR1, pH 8.0, temperature not specified in the publication | Pseudomonas aeruginosa | |
0.3 | - |
Methyl red | isoform AzoR2, pH 8.0, temperature not specified in the publication | Pseudomonas aeruginosa | |
0.34 | - |
balsalzide | isoform AzoR3, pH 8.0, temperature not specified in the publication | Pseudomonas aeruginosa | |
0.38 | - |
sulfasalazine | isoform AzoR2, pH 8.0, temperature not specified in the publication | Pseudomonas aeruginosa | |
0.525 | - |
NADH | isoform AzoR1, pH 8.0, temperature not specified in the publication | Pseudomonas aeruginosa | |
0.983 | - |
Methyl red | isoform AzoR3, pH 8.0, temperature not specified in the publication | Pseudomonas aeruginosa | |
1.118 | - |
sulfasalazine | isoform AzoR1, pH 8.0, temperature not specified in the publication | Pseudomonas aeruginosa | |
2.447 | - |
balsalzide | isoform AzoR1, pH 8.0, temperature not specified in the publication | Pseudomonas aeruginosa | |
10.26 | - |
NADPH | isoform AzoR1, pH 8.0, temperature not specified in the publication | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
N,N-dimethyl-1,4-phenylenediamine + aniline + 2 NADP+ = 4-(dimethylamino)azobenzene + 2 NADPH + 2 H+ | ping-pong mechanism | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
balsalzide + NADH + H+ | - |
Pseudomonas aeruginosa | ? + NAD+ | - |
? | |
methyl red + NADPH + H+ | - |
Pseudomonas aeruginosa | ? + NADP+ | - |
? | |
additional information | no substrate: azobenzene | Pseudomonas aeruginosa | ? | - |
? | |
sulfasalazine + NADPH + H+ | - |
Pseudomonas aeruginosa | ? + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AzoR1 | - |
Pseudomonas aeruginosa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
balsalzide | isoform AzoR3, pH 8.0, temperature not specified in the publication | Pseudomonas aeruginosa | |
0.5 | - |
sulfasalazine | isoform AzoR3, pH 8.0, temperature not specified in the publication | Pseudomonas aeruginosa | |
0.7 | - |
balsalzide | isoform AzoR2, pH 8.0, temperature not specified in the publication | Pseudomonas aeruginosa | |
0.8 | - |
sulfasalazine | isoform AzoR2, pH 8.0, temperature not specified in the publication | Pseudomonas aeruginosa | |
1 | - |
Methyl red | isoform AzoR2, pH 8.0, temperature not specified in the publication | Pseudomonas aeruginosa | |
2.6 | - |
sulfasalazine | isoform AzoR1, pH 8.0, temperature not specified in the publication | Pseudomonas aeruginosa | |
4.8 | - |
Methyl red | isoform AzoR1, pH 8.0, temperature not specified in the publication | Pseudomonas aeruginosa | |
11.6 | - |
Methyl red | isoform AzoR3, pH 8.0, temperature not specified in the publication | Pseudomonas aeruginosa | |
20 | - |
balsalzide | isoform AzoR1, pH 8.0, temperature not specified in the publication | Pseudomonas aeruginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | - |
Pseudomonas aeruginosa | |
NADH | isoforms AzoR2 and AzoR3 prefer NADH over NADPH | Pseudomonas aeruginosa | |
NADPH | isoform AzoR1 prefers NADPH over NADH | Pseudomonas aeruginosa |
Organism | Comment | Expression |
---|---|---|
Pseudomonas aeruginosa | in presence of methyl red, expression of isoform AzoR1 increases more than 50% | down |
Pseudomonas aeruginosa | in presence of balsalazide, expression of isoform AzoR1 increases by about 50% | up |
Pseudomonas aeruginosa | in presence of methyl red or balsalzide, expression of isoform AzoR2 increases 3.5- and 2.5fold, respectively | up |