Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Shewanella oneidensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | 5 mM, 30% residual activity | Shewanella oneidensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0028 | - |
Methyl red | cosubstrate NADPH, pH 6.5, 25°C | Shewanella oneidensis | |
0.0092 | - |
Methyl red | cosubstrate NADH, pH 6.5, 25°C | Shewanella oneidensis | |
0.48 | - |
NADH | pH 6.5, 25°C | Shewanella oneidensis | |
0.941 | - |
NADPH | pH 6.5, 25°C | Shewanella oneidensis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activation, optimum concentration 25 mM giving 126% of initial activity | Shewanella oneidensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
x * 27000, SDS-PAGE | Shewanella oneidensis |
Organic Solvent | Comment | Organism |
---|---|---|
Acetone | 30%, no significant loss of activity | Shewanella oneidensis |
dimethyl sulfoxide | 30%, no significant loss of activity | Shewanella oneidensis |
Ethanol | 20%, no significant loss of activity | Shewanella oneidensis |
isopropanol | 20%, no significant loss of activity | Shewanella oneidensis |
Methanol | 30%, no significant loss of activity | Shewanella oneidensis |
propanol | 10%, no significant loss of activity | Shewanella oneidensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Shewanella oneidensis | Q8E990 | - |
- |
Shewanella oneidensis MR-1 / ATCC 700550 | Q8E990 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
156.1 | - |
pH 6.5, 25°C | Shewanella oneidensis |
184.6 | - |
presence of 25 microM FMN, pH 6.5, 25°C | Shewanella oneidensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methyl red + NADH + H+ | - |
Shewanella oneidensis | ? + NAD+ | - |
? | |
methyl red + NADH + H+ | - |
Shewanella oneidensis MR-1 / ATCC 700550 | ? + NAD+ | - |
? | |
methyl red + NADPH + H+ | - |
Shewanella oneidensis | ? + NADP+ | - |
? | |
methyl red + NADPH + H+ | - |
Shewanella oneidensis MR-1 / ATCC 700550 | ? + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 27000, SDS-PAGE | Shewanella oneidensis |
Synonyms | Comment | Organism |
---|---|---|
AzoR | - |
Shewanella oneidensis |
SO 4396 | - |
Shewanella oneidensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Shewanella oneidensis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7.5 | more than 50% of maximum activity | Shewanella oneidensis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | - |
Shewanella oneidensis | |
NADH | preferred cofactor | Shewanella oneidensis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
30 | - |
NADPH | pH 6.5, 25°C | Shewanella oneidensis | |
210 | - |
NADH | pH 6.5, 25°C | Shewanella oneidensis | |
6760 | - |
Methyl red | cosubstrate NADPH, pH 6.5, 25°C | Shewanella oneidensis | |
8970 | - |
Methyl red | cosubstrate NADH, pH 6.5, 25°C | Shewanella oneidensis |