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Literature summary for 1.7.1.6 extracted from

  • Ooi, T.; Shibata, T.; Matsumoto, K.; Kinoshita, S.; Taguchi, S.
    Comparative enzymatic analysis of azoreductases from bacillus sp. B29 (2009), Biosci. Biotechnol. Biochem., 73, 1209-1211.
    View publication on PubMed
Search for more literature for 1.7.1.6

Application

Application Comment Organism
industry azoreductases are favorable for the development of biodegradation process for azo dyes Bacillus sp. B29

Cloned(Commentary)

Cloned (Comment) Organism
into the vector pGEM-T and subsequently into pET3a for expression in Escherichia coli BL21DE3 cells Bacillus sp. B29

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0364
-
 NADH
-
 Bacillus sp. B29
0.037
-
 Methyl red
-
 Bacillus sp. B29
0.0611
-
 Methyl red
-
 Bacillus sp. B29
0.105
-
 NADH
-
 Bacillus sp. B29

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22900
-
-
 Bacillus sp. B29
23000
-
-
 Bacillus sp. B29

Organism

Organism UniProt Comment Textmining
Bacillus sp. B29 C0STY0
-
-
Bacillus sp. B29 C0STY1
-
-

Purification (Commentary)

Purification (Comment) Organism
crude cell extracts are applied on a DEAE cellulose column, subsequently to an ammonium sulfate precipitation the enzyme solution is applied on a Butyl Toyopearl 650M column, finally a Sephacryl S200 column is used Bacillus sp. B29
crude cell extracts are applied on a DEAE cellulose column, subsequently to an ammonium sulfate precipitation the enzyme solution is applied on a Phenyl Toyopearl 650M column, finally a Sephacryl S200 column is used Bacillus sp. B29

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 substrate 1-(2-pyridylazo)-2-naphthol, relative activity 0.64%, methyl red 100% Bacillus sp. B29
additional information
-
 substrate 1-(2-pyridylazo)-2-naphthol, relative activity 23.2%, methyl red 100% Bacillus sp. B29
additional information
-
 substrate acid red 88, relative activity 0.12%, methyl red 100% Bacillus sp. B29
additional information
-
 substrate acid red 88, relative activity 12.2%, methyl red 100% Bacillus sp. B29
additional information
-
 substrate ethyl red, relative activity 113%, methyl red 100% Bacillus sp. B29
additional information
-
 substrate ethyl red, relative activity 146%, methyl red 100% Bacillus sp. B29
additional information
-
 substrate methyl orange, relative activity 0.21%, methyl red 100% Bacillus sp. B29
additional information
-
 substrate methyl orange, relative activity 0.93%, methyl red 100% Bacillus sp. B29
additional information
-
 substrate methyl red, relative activity 100% Bacillus sp. B29
additional information
-
 substrate new cossin, relative activity 0.1%, methyl red 100% Bacillus sp. B29
additional information
-
 substrate orange I, relative activity 0.03%, methyl red 100% Bacillus sp. B29
additional information
-
 substrate orange I, relative activity 1.26%, methyl red 100% Bacillus sp. B29
additional information
-
 substrate orange II, relative activity 0.01%, methyl red 100% Bacillus sp. B29
additional information
-
 substrate orange II, relative activity 0.29%, methyl red 100% Bacillus sp. B29
additional information
-
 substrate sunset yellow FCF, relative activity 0.16%, methyl red 100% Bacillus sp. B29
38.2
-
 substrate methyl red Bacillus sp. B29
38.6
-
 substrate methyl red Bacillus sp. B29

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-(2-pyridylazo)-2-naphthol + NADH + H+
-
 Bacillus sp. B29 ?
-
 ?
acid red 88 + NADH + H+
-
 Bacillus sp. B29 ?
-
 ?
ethyl red + NADH + H+
-
 Bacillus sp. B29 ?
-
 ?
methyl orange + NADH + H+
-
 Bacillus sp. B29 ?
-
 ?
methyl red + NADH + H+
-
 Bacillus sp. B29 ?
-
 ?
new cossin + NADH + H+
-
 Bacillus sp. B29 ?
-
 ?
orange I + NADH + H+
-
 Bacillus sp. B29 ?
-
 ?
orange II + NADH + H+
-
 Bacillus sp. B29 ?
-
 ?
sunset yellow FCF + NADH + H+
-
 Bacillus sp. B29 ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 22900, determined by gel filtration Bacillus sp. B29
homodimer 2 * 23000, determined by gel filtration Bacillus sp. B29

Synonyms

Synonyms Comment Organism
azoreductase B
-
 Bacillus sp. B29
azoreductase C
-
 Bacillus sp. B29
AzrB
-
 Bacillus sp. B29
AzrC
-
 Bacillus sp. B29

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Bacillus sp. B29
70
-
-
 Bacillus sp. B29

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 activity assay Bacillus sp. B29

pH Range

pH Minimum pH Maximum Comment Organism
5 11
-
 Bacillus sp. B29
6.5 10
-
 Bacillus sp. B29

Cofactor

Cofactor Comment Organism Structure
FMN
-
 Bacillus sp. B29
NADH
-
 Bacillus sp. B29