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Literature summary for 1.7.1.6 extracted from

  • Smith, E.J.; Van Loon, E.J.
    1,2-Dimethyl-4-(p-carboxyphenylazo)-5-hydroxybenzene. A convenient substrate for the measurement of azo reductase (1969), Anal. Biochem., 31, 315-320.
    View publication on PubMed
Search for more literature for 1.7.1.6

Activating Compound

Activating Compound Comment Organism Structure
furazolidone
-
 Rattus norvegicus
Nitrofurantoin
-
 Rattus norvegicus
nitrofurazone
-
 Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
 1,2-dimethyl-4-(p-carboxyphenylazo)-5-hydroxy-benzene
-
 Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Canis lupus familiaris
-
-
-
Cavia porcellus
-
-
-
Gallus gallus
-
-
-
Microtus pennsylvanicus
-
 meadow vole
-
Rattus norvegicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Gallus gallus
-
liver
-
 Cavia porcellus
-
liver
-
 Rattus norvegicus
-
liver
-
 Canis lupus familiaris
-
liver
-
 Microtus pennsylvanicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.045
-
 activity in liver supernatant fraction Canis lupus familiaris
0.053
-
 activity in liver supernatant fraction Gallus gallus
0.06
-
 activity in liver supernatant fraction Microtus pennsylvanicus
0.067
-
 activity in liver supernatant fraction Rattus norvegicus
0.087
-
 activity in liver supernatant fraction Cavia porcellus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-dimethyl-4(p-carboxyphenylazo)-5-hydroxybenzene + NADPH
-
 Gallus gallus 1,2-dimethyl-4-amino-5-hydroxybenzene + p-carboxyphenylamine + NADP+
-
 ?
1,2-dimethyl-4(p-carboxyphenylazo)-5-hydroxybenzene + NADPH
-
 Cavia porcellus 1,2-dimethyl-4-amino-5-hydroxybenzene + p-carboxyphenylamine + NADP+
-
 ?
1,2-dimethyl-4(p-carboxyphenylazo)-5-hydroxybenzene + NADPH
-
 Rattus norvegicus 1,2-dimethyl-4-amino-5-hydroxybenzene + p-carboxyphenylamine + NADP+
-
 ?
1,2-dimethyl-4(p-carboxyphenylazo)-5-hydroxybenzene + NADPH
-
 Canis lupus familiaris 1,2-dimethyl-4-amino-5-hydroxybenzene + p-carboxyphenylamine + NADP+
-
 ?
1,2-dimethyl-4(p-carboxyphenylazo)-5-hydroxybenzene + NADPH
-
 Microtus pennsylvanicus 1,2-dimethyl-4-amino-5-hydroxybenzene + p-carboxyphenylamine + NADP+
-
 ?
4-(dimethylamino)azobenzene + 2 NADPH
-
 Gallus gallus N,N-dimethyl-1,4-phenylenediamine + aniline + 2 NADP+ + 2 H+
-
 ?
4-(dimethylamino)azobenzene + 2 NADPH
-
 Cavia porcellus N,N-dimethyl-1,4-phenylenediamine + aniline + 2 NADP+ + 2 H+
-
 ?
4-(dimethylamino)azobenzene + 2 NADPH
-
 Rattus norvegicus N,N-dimethyl-1,4-phenylenediamine + aniline + 2 NADP+ + 2 H+
-
 ?
4-(dimethylamino)azobenzene + 2 NADPH
-
 Canis lupus familiaris N,N-dimethyl-1,4-phenylenediamine + aniline + 2 NADP+ + 2 H+
-
 ?
4-(dimethylamino)azobenzene + 2 NADPH
-
 Microtus pennsylvanicus N,N-dimethyl-1,4-phenylenediamine + aniline + 2 NADP+ + 2 H+
-
 ?

Cofactor

Cofactor Comment Organism Structure
FAD increases activity Rattus norvegicus