Activating Compound | Comment | Organism | Structure |
---|---|---|---|
FMN | enzyme contains no FMN | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
NADPH | - |
Rattus norvegicus | |
0.32 | - |
Methyl red | - |
Rattus norvegicus | |
0.4 | - |
NADH | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rattus norvegicus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
2 * 30000, SDS-PAGE | Rattus norvegicus |
52000 | - |
gel filtration | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
17.83 | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-carboxy-4-N,N-dimethylazobenzene + NAD(P)H | trivial name methyl red | Rattus norvegicus | 2-carboxy-1-phenylamine + N,N-dimethyl-1,4-phenylenediamine + NADP+ | - |
? | |
4-(dimethylamino)azobenzene + 2 NADPH | specific for reduction of methyl red | Rattus norvegicus | N,N-dimethyl-1,4-phenylenediamine + aniline + 2 NADP+ + 2 H+ | - |
? | |
methyl red + 2 NADH + 2 H+ | - |
Rattus norvegicus | 2-aminobenzoic acid + N,N-dimethyl-1,4-phenylenediamine + 2 NAD+ | - |
r | |
methyl red + NADH + H+ | - |
Rattus norvegicus | ? | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 30000, SDS-PAGE | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | 6 | - |
Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 7 | pH 4.0: about 25% activity, pH 7.0: about 20% activity | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | one mol of enzyme contains 2 mol of FAD | Rattus norvegicus | |
flavin | typical flavoprotein absorption spectrum | Rattus norvegicus | |
NADH | - |
Rattus norvegicus | |
NADPH | - |
Rattus norvegicus |