Protein Variants | Comment | Organism |
---|---|---|
additional information | nit-1 mutant, suggested to produce the complete apoenzyme | Neurospora crassa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.022 | 0.05 | NADPH | - |
Neurospora crassa | |
0.29 | - |
nitrate | - |
Neurospora crassa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Molybdenum | - |
Neurospora crassa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
145000 | - |
monodimer, 2 * 145000 | Neurospora crassa |
290000 | - |
- |
Neurospora crassa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrate + NADPH | Neurospora crassa | - |
nitrite + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neurospora crassa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
protamine sulfate and ammonium sulfate precipitation, hemoglobin-Sepharose column, Bio-Gel A, FAD-Sepharose affinity column. proteolysis during extensive purification | Neurospora crassa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.063 | - |
nit-1 mutant | Neurospora crassa |
1.24 | - |
wild-type | Neurospora crassa |
Storage Stability | Organism |
---|---|
-10°C, 0.1 M sodium phosphate buffer, pH 7.3, 0.17 M NaCl, 1 mM dithiothreitol, 5 m M EDTA, 0.5 mM PMSF, 1%ethanol, 30% glycerol | Neurospora crassa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrate + NADPH | - |
Neurospora crassa | nitrite + NADP+ + H2O | - |
? | |
nitrate + NADPH | 4 activities: NADPH-nitrate reductase, FADH-nitrate reductase, reduced methyl viologen-nitrate reductase and NADPH-cytochrome c reductase | Neurospora crassa | nitrite + NADP+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | monodimer, 2 * 145000 | Neurospora crassa |
More | nit-1 mutant enzyme is the apoprotein of nitrate reductase | Neurospora crassa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Neurospora crassa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
49 | - |
50% loss of activity after 1.5 min | Neurospora crassa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
assay at | Neurospora crassa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Neurospora crassa |