Any feedback?
Literature summary for 1.7.1.3 extracted from
- Triphosphopyridine nucleotide-nitrate reductase in Neurospora (1953), J. Biol. Chem., 202, 655-673.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 8-hydroxyquinoline | - |
Neurospora crassa |  |
| azide | - |
Neurospora crassa | |
| Cu2+ | inhibitory | Neurospora crassa | |
| cyanide | - |
Neurospora crassa | |
| o-phenanthroline | - |
Neurospora crassa | |
| p-chloromercuribenzoate | reversed by cysteine or glutathione | Neurospora crassa | |
| potassium ethyl xanthate | - |
Neurospora crassa | |
| Thiourea | - |
Neurospora crassa | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nitrate + NADPH | Neurospora crassa | - |
nitrite + NADP+ + H2O | - |
? | |
| nitrate + NADPH | Neurospora crassa 5297a | - |
nitrite + NADP+ + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Neurospora crassa | - |
5297a | - |
| Neurospora crassa 5297a | - |
5297a | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation | Neurospora crassa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | - |
Neurospora crassa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
one unit is defined as that amount of enzyme which results in the formation of 0.000001 mM of nitrite | Neurospora crassa |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -15°C, fraction II, most stable, optimal pH: 7.0, 10-20% loss of activity after a month | Neurospora crassa |
| -15°C, fraction IV, one week, loses about half of its activity | Neurospora crassa |
| -15°C, fraction V, quite unstable, overnight, loses at least half of its activity | Neurospora crassa |
| 4°C or -15°C, fraction III, overnight, 50% loss of activity | Neurospora crassa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nitrate + NADPH | - |
Neurospora crassa | nitrite + NADP+ + H2O | - |
? | |
| nitrate + NADPH | 20-fold higher activity with NADPH than with NADH | Neurospora crassa | nitrite + NADP+ + H2O | - |
ir | |
| nitrate + NADPH | - |
Neurospora crassa 5297a | nitrite + NADP+ + H2O | - |
? | |
| nitrate + NADPH | 20-fold higher activity with NADPH than with NADH | Neurospora crassa 5297a | nitrite + NADP+ + H2O | - |
ir | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 23 | 28 | assay at | Neurospora crassa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
fraction V, pH 7.0, 50% loss of activity within 5 min | Neurospora crassa |
| 50 | - |
fraction V, pH 7.0, loses all of its activity in 5 min | Neurospora crassa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Neurospora crassa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | - |
Neurospora crassa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | flavoprotein, prosthetic group | Neurospora crassa | |
| FAD | stimulated by flavin | Neurospora crassa | |
| FMN | to a lesser extent than FAD | Neurospora crassa | |
| additional information | cytochrome a or b involved | Neurospora crassa |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
