Inhibitors | Comment | Organism | Structure |
---|---|---|---|
8-hydroxyquinoline | - |
Neurospora crassa | |
azide | - |
Neurospora crassa | |
Cu2+ | inhibitory | Neurospora crassa | |
cyanide | - |
Neurospora crassa | |
o-phenanthroline | - |
Neurospora crassa | |
p-chloromercuribenzoate | reversed by cysteine or glutathione | Neurospora crassa | |
potassium ethyl xanthate | - |
Neurospora crassa | |
Thiourea | - |
Neurospora crassa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrate + NADPH | Neurospora crassa | - |
nitrite + NADP+ + H2O | - |
? | |
nitrate + NADPH | Neurospora crassa 5297a | - |
nitrite + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neurospora crassa | - |
5297a | - |
Neurospora crassa 5297a | - |
5297a | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation | Neurospora crassa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Neurospora crassa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
one unit is defined as that amount of enzyme which results in the formation of 0.000001 mM of nitrite | Neurospora crassa |
Storage Stability | Organism |
---|---|
-15°C, fraction II, most stable, optimal pH: 7.0, 10-20% loss of activity after a month | Neurospora crassa |
-15°C, fraction IV, one week, loses about half of its activity | Neurospora crassa |
-15°C, fraction V, quite unstable, overnight, loses at least half of its activity | Neurospora crassa |
4°C or -15°C, fraction III, overnight, 50% loss of activity | Neurospora crassa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrate + NADPH | - |
Neurospora crassa | nitrite + NADP+ + H2O | - |
? | |
nitrate + NADPH | 20-fold higher activity with NADPH than with NADH | Neurospora crassa | nitrite + NADP+ + H2O | - |
ir | |
nitrate + NADPH | - |
Neurospora crassa 5297a | nitrite + NADP+ + H2O | - |
? | |
nitrate + NADPH | 20-fold higher activity with NADPH than with NADH | Neurospora crassa 5297a | nitrite + NADP+ + H2O | - |
ir |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | 28 | assay at | Neurospora crassa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
fraction V, pH 7.0, 50% loss of activity within 5 min | Neurospora crassa |
50 | - |
fraction V, pH 7.0, loses all of its activity in 5 min | Neurospora crassa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Neurospora crassa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | - |
Neurospora crassa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | flavoprotein, prosthetic group | Neurospora crassa | |
FAD | stimulated by flavin | Neurospora crassa | |
FMN | to a lesser extent than FAD | Neurospora crassa | |
additional information | cytochrome a or b involved | Neurospora crassa |