General Stability | Organism |
---|---|
loss of activity with ammonium chloride or ammonium nitrate at 10 mM to growing cells, faster decay in washed and resuspended cells | Funaria hygrometrica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | 0.015 | nitrate | - |
Funaria hygrometrica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
NH4+ | represses the intracellular NO3- assimilation, which is required for the induction and maintenace of NADPH nitrate reductase, enzyme is regulated by NH4+ at the transcriptional level | Funaria hygrometrica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
116000 | - |
4 * 116000 | Funaria hygrometrica |
450000 | - |
native gel electrophoresis | Funaria hygrometrica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrate + NADPH | Funaria hygrometrica | - |
nitrite + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Funaria hygrometrica | - |
Hedw. | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
chloronema | - |
Funaria hygrometrica | - |
Storage Stability | Organism |
---|---|
half life of about 2 h with ammonium sulfate or ammonium chloride as sole N-source | Funaria hygrometrica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrate + NADPH | - |
Funaria hygrometrica | nitrite + NADP+ + H2O | - |
? | |
nitrate + NADPH | associated cytochrome c reductase activity | Funaria hygrometrica | nitrite + NADP+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 116000 | Funaria hygrometrica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Funaria hygrometrica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Funaria hygrometrica |