Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.7.1.2 extracted from

  • De La Rosa, M.A.; Diez, J.; Vega, J.M.; Losada, M.
    Purification and properties of assimilatory nitrate reductase [NAD(P)H] from Ankistrodesmus braunii (1980), Eur. J. Biochem., 106, 249-256.
    View publication on PubMed
Search for more literature for 1.7.1.2

Inhibitors

Inhibitors Comment Organism Structure
p-hydroxymercuribenzoate
-
 Ankistrodesmus braunii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
460000 467400 Ankistrodesmus braunii, gel filtration, density gradient centrifugation, disc gel electrophoresis, pore gradient electrophoresis, sucrose-density gradient centrifugation Ankistrodesmus braunii

Organism

Organism UniProt Comment Textmining
Ankistrodesmus braunii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Ankistrodesmus braunii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
61.25
-
-
 Ankistrodesmus braunii
72 80
-
 Ankistrodesmus braunii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NAD(P)H + nitrate
-
 Ankistrodesmus braunii NAD(P)+ + nitrite + H2O
-
 ?

Subunits

Subunits Comment Organism
octamer 8 * 58750-59000, Ankistrodesmus braunii, SDS-PAGE Ankistrodesmus braunii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
470
-
 NADPH
-
 Ankistrodesmus braunii

Cofactor

Cofactor Comment Organism Structure
heme minimum of 4 molecules of heme per molecule of native enzyme Ankistrodesmus braunii
NADH NADPH to NADH activity ratio is 1.8 Ankistrodesmus braunii
NADPH NADPH to NADH activity ratio is 1.8 Ankistrodesmus braunii