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Literature summary for 1.7.1.14 extracted from
- Purification and functional analysis of fungal nitric oxide reductase cytochrome P450nor (2008), Methods Enzymol., 437, 117-133.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli JM109(DE3) cells | Cutaneotrichosporon cutaneum |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| S73G/S75GS75G | the mutant exhibits 79% activity of wild type with NADH | Fusarium oxysporum |
| S75G | the mutant exhibits 63% activity of wild type with NADH | Fusarium oxysporum |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Cutaneotrichosporon cutaneum | 5739 | - |
| mitochondrion | - |
Aspergillus oryzae | 5739 | - |
| mitochondrion | - |
Fusarium oxysporum | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45200 | - |
calculated from amino acid sequence | Cutaneotrichosporon cutaneum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus oryzae | - |
- |
- |
| Cutaneotrichosporon cutaneum | - |
- |
- |
| Fusarium lichenicola | - |
- |
- |
| Fusarium oxysporum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE cellulose column chromatography, phenyl-Superose column chromatography, and Mono Q column chromatography or Resource Q column chromatography | Cutaneotrichosporon cutaneum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | P450nor is a reductase without monooxygenase activity | Cutaneotrichosporon cutaneum | ? | - |
? |  |
| additional information | P450nor is a reductase without monooxygenase activity | Aspergillus oryzae | ? | - |
? | |
| additional information | P450nor is a reductase without monooxygenase activity | Fusarium oxysporum | ? | - |
? | |
| additional information | P450nor is a reductase without monooxygenase activity | Fusarium lichenicola | ? | - |
? | |
| NO + NADH + H+ | - |
Cutaneotrichosporon cutaneum | N2O + NAD+ + H2O | - |
? | |
| NO + NADH + H+ | - |
Aspergillus oryzae | N2O + NAD+ + H2O | - |
? | |
| NO + NADH + H+ | - |
Fusarium lichenicola | N2O + NAD+ + H2O | - |
? | |
| NO + NADH + H+ | Fnor is specific for NADH | Fusarium oxysporum | N2O + NAD+ + H2O | - |
? | |
| NO + NADPH + H+ | - |
Cutaneotrichosporon cutaneum | N2O + NADP+ + H2O | - |
? | |
| NO + NADPH + H+ | - |
Aspergillus oryzae | N2O + NADP+ + H2O | - |
? | |
| NO + NADPH + H+ | - |
Fusarium lichenicola | N2O + NADP+ + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Anor | - |
Aspergillus oryzae |
| Cnor1 | isozyme | Fusarium lichenicola |
| Cnor2 | isozyme | Fusarium lichenicola |
| CYP55A1 | - |
Fusarium oxysporum |
| CYP55A2 | - |
Fusarium lichenicola |
| CYP55A3 | - |
Fusarium lichenicola |
| CYP55A4 | - |
Cutaneotrichosporon cutaneum |
| CYP55A5 | - |
Aspergillus oryzae |
| cytochrome P450nor | - |
Cutaneotrichosporon cutaneum |
| cytochrome P450nor | - |
Aspergillus oryzae |
| cytochrome P450nor | - |
Fusarium oxysporum |
| cytochrome P450nor | - |
Fusarium lichenicola |
| Fnor | - |
Fusarium oxysporum |
| fungal nitric oxide reductase | - |
Cutaneotrichosporon cutaneum |
| fungal nitric oxide reductase | - |
Aspergillus oryzae |
| fungal nitric oxide reductase | - |
Fusarium oxysporum |
| fungal nitric oxide reductase | - |
Fusarium lichenicola |
| P450nor | - |
Cutaneotrichosporon cutaneum |
| P450nor | - |
Aspergillus oryzae |
| P450nor | - |
Fusarium oxysporum |
| P450nor | - |
Fusarium lichenicola |
| Tnor | - |
Cutaneotrichosporon cutaneum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme | heme-thiolate protein | Cutaneotrichosporon cutaneum | |
| heme | heme-thiolate protein | Aspergillus oryzae | |
| heme | heme-thiolate protein | Fusarium oxysporum | |
| heme | heme-thiolate protein | Fusarium lichenicola | |
| NADH | Anor can utilize both NADH and NADPH | Aspergillus oryzae | |
| NADH | Fnor is specific for NADH | Fusarium oxysporum | |
| NADH | isozyme Cnor1 is specific for NADH | Fusarium lichenicola | |
| NADH | Tnor can utilize both NADH and NADPH | Cutaneotrichosporon cutaneum | |
| NADPH | Anor can utilize both NADH and NADPH | Aspergillus oryzae | |
| NADPH | isozyme Cnor2 can utilize both NADH and NADPH | Fusarium lichenicola | |
| NADPH | Nor activity of wild type with NADPH as electron donor is only 13% of that with NADH as electron donor | Fusarium oxysporum | |
| NADPH | Tnor can utilize both NADH and NADPH | Cutaneotrichosporon cutaneum | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | P450nor is involved in fungal denitrification, it receives electrons directly from the distal pocket from NADH and/or NADPH without any help from a flavoprotein | Cutaneotrichosporon cutaneum |
| physiological function | P450nor is involved in fungal denitrification, it receives electrons directly from the distal pocket from NADH and/or NADPH without any help from a flavoprotein | Aspergillus oryzae |
| physiological function | P450nor is involved in fungal denitrification, it receives electrons directly from the distal pocket from NADH and/or NADPH without any help from a flavoprotein | Fusarium oxysporum |
| physiological function | P450nor is involved in fungal denitrification, it receives electrons directly from the distal pocket from NADH and/or NADPH without any help from a flavoprotein | Fusarium lichenicola |
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