KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.801 | - |
NADH | in 50 mM sodium phosphate (pH 7.2), at 37°C | Aspergillus oryzae | |
0.934 | - |
NADPH | in 50 mM sodium phosphate (pH 7.2), at 37°C | Aspergillus oryzae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
SDS-PAGE | Aspergillus oryzae |
45690 | - |
deduced from amino acid sequence | Aspergillus oryzae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus oryzae | Q8NKB4 | - |
- |
Aspergillus oryzae OSI 1013 | Q8NKB4 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Resource Q column chromatography, Resource PHE column chromatography, and Superdex 200 gel filtration | Aspergillus oryzae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NO + NADH + H+ | - |
Aspergillus oryzae | N2O + NAD+ + H2O | - |
? | |
NO + NADH + H+ | - |
Aspergillus oryzae OSI 1013 | N2O + NAD+ + H2O | - |
? | |
NO + NADPH + H+ | - |
Aspergillus oryzae | N2O + NADP+ + H2O | - |
? | |
NO + NADPH + H+ | - |
Aspergillus oryzae OSI 1013 | N2O + NADP+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Anor | - |
Aspergillus oryzae |
cytochrome P450nor | - |
Aspergillus oryzae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Aspergillus oryzae | |
NADH | the enzyme employs both NADH and NADPH as electron donors | Aspergillus oryzae | |
NADPH | the enzyme employs both NADH and NADPH as electron donors | Aspergillus oryzae |