Literature summary for 1.7.1.13 extracted from

Kim, Y.; Zhou, M.; Moy, S.; Morales, J.; Cunningham, M.A.; Joachimiak, A.
High-resolution structure of the nitrile reductase QueF combined with molecular simulations provide insight into enzyme mechanism (2010), J. Mol. Biol., 404, 127-137.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method, using 0.04 M sodium dihydrogen phosphate, 0.96 M dipotassium hydrogen phosphate, and 10 mM GTP	Vibrio cholerae

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
33000	-	2 * 33000, gel filtration	Vibrio cholerae
67900	-	gel filtration	Vibrio cholerae

Organism

Organism	UniProt	Comment	Textmining
Vibrio cholerae	Q9KTK0	-	-
Vibrio cholerae ATCC 39315	Q9KTK0	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA agarose column chromatography	Vibrio cholerae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
7-cyano-7-deazaguanine + 2 NADPH + 2 H+	-	Vibrio cholerae	queuine + 2 NADP+	queuine is 7-aminomethyl-7-deazaguanine	?
7-cyano-7-deazaguanine + 2 NADPH + 2 H+	-	Vibrio cholerae ATCC 39315	queuine + 2 NADP+	queuine is 7-aminomethyl-7-deazaguanine	?

Subunits

Subunits	Comment	Organism
homodimer	2 * 33000, gel filtration	Vibrio cholerae

Synonyms

Synonyms	Comment	Organism
7-cyano-7-deazaguanine reductase	-	Vibrio cholerae
nitrile reductase	-	Vibrio cholerae
QueF	-	Vibrio cholerae

Cofactor

Cofactor	Comment	Organism	Structure
NADPH	-	Vibrio cholerae

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Release 2023.1 (January 2023)