Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,2'-dipyridyl | 1 mM, 84% inhibition | Glycine max | |
8-hydroxyquinoline | 1 mM, complete inhibition | Glycine max | |
hydrazine | 1 mM, complete inhibition | Glycine max | |
KCN | 0.1 mM, complete inhibition | Glycine max | |
NaN3 | 1 mM, complete inhibition | Glycine max | |
o-phenanthroline | 1 mM, complete inhibition | Glycine max | |
p-chloromercuribenzoate | 0.1 mM, complete inhibition | Glycine max | |
potassium ethyl xanthate | - |
Glycine max | |
Salicylaldoxime | - |
Glycine max | |
Sodium diethyl dithiocarbamate | 1 mM, complete inhibition | Glycine max |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Glycine max | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | 0.1 mM, approx. 6fold stimulation | Glycine max |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | - |
soybean | - |
Purification (Comment) | Organism |
---|---|
enzyme not seperated from pyridine nucleotide-nitrite reductase | Glycine max |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Glycine max | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
794.1 units/mg, 1 unit is defined as that amount of enzyme which causes a rate of change in extinction of 0.001 per minute | Glycine max |
Storage Stability | Organism |
---|---|
-20°C, 1 month, 60-90% loss of activity | Glycine max |
4°C, 1 week, no loss in activity | Glycine max |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hydroxylamine + NADH | NADH is 1.5fold more effective than NADPH | Glycine max | NH3 + NAD+ + H2O | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
complete loss of activity after 5 min | Glycine max |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.6 | 7.1 | - |
Glycine max |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | purified enzyme: absolute requirement for an unidentified, dissociable, heat-stable, organic factor obtained from soybean leaf extract, no flavin requirement | Glycine max | |
NADH | - |
Glycine max | |
NADPH | about 65% of the activity with NADH | Glycine max |