Cloned (Comment) | Organism |
---|---|
the C-terminal 268 residues corresponding to the flavin-containing domain, amplified and expressed in Escherichia coli | Spinacia oleracea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
adenine | inhibition of the recombinant FAD domain | Spinacia oleracea | |
adenosine | inhibition of the recombinant FAD domain | Spinacia oleracea | |
adenosine 5'-diphosphoribose | inhibition of the recombinant FAD domain | Spinacia oleracea | |
ADP | inhibition of the recombinant FAD domain | Spinacia oleracea | |
AMP | inhibition of the recombinant FAD domain | Spinacia oleracea | |
ferricyanide | inhibition of the recombinant FAD domain | Spinacia oleracea | |
NAD+ | inhibition of the recombinant FAD domain | Spinacia oleracea | |
nicotinamide | inhibition of the recombinant FAD domain | Spinacia oleracea | |
NMN | inhibition of the recombinant FAD domain | Spinacia oleracea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km-values of the the C-terminal 268 residues corresponding to the flavin-containing domain, amplified and expressed in E. coli | Spinacia oleracea | |
0.017 | - |
NADH | recombinant FAD domain | Spinacia oleracea | |
0.034 | - |
ferricyanide | recombinant FAD domain | Spinacia oleracea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
the C-terminal 268 residues corresponding to the flavin-containing domain, amplified and expressed in E. coli show a MW of 30000 Da by SDS-PAGE | Spinacia oleracea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Spinacia oleracea | - |
- |
- |
Purification (Comment) | Organism |
---|---|
the C-terminal 268 residues corresponding to the flavin-containing domain, amplified and expressed in Escherichia coli | Spinacia oleracea |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Spinacia oleracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the isolated flavin-containing domain is capable of reducing cytochrome b5 directly | Spinacia oleracea | ? | - |
? | |
nitrate + NADH | - |
Spinacia oleracea | nitrite + NAD+ + H2O | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Spinacia oleracea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Spinacia oleracea |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
ADP | inhibition of the recombinant FAD domain | Spinacia oleracea | |
0.2 | - |
ADPribose | inhibition of the recombinant FAD domain | Spinacia oleracea | |
0.6 | - |
AMP | inhibition of the recombinant FAD domain | Spinacia oleracea | |
1.9 | - |
NAD+ | inhibition of the recombinant FAD domain | Spinacia oleracea | |
4.8 | - |
adenosine | inhibition of the recombinant FAD domain | Spinacia oleracea | |
23 | - |
ferricyanide | inhibition of the recombinant FAD domain | Spinacia oleracea | |
26 | - |
adenine | inhibition of the recombinant FAD domain | Spinacia oleracea | |
62 | - |
NMN | inhibition of the recombinant FAD domain | Spinacia oleracea | |
122 | - |
nicotinamide | inhibition of the recombinant FAD domain | Spinacia oleracea |