KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.052 | - |
NADH | reaction with nitrate | Rhodobacter capsulatus | |
0.067 | - |
NADH | reaction with 2,6-dichlorophenol indophenol | Rhodobacter capsulatus | |
0.096 | - |
nitrate | reaction with NADH | Rhodobacter capsulatus | |
0.13 | - |
nitrate | reaction with methyl viologen | Rhodobacter capsulatus | |
0.54 | - |
reduced bromophenol blue | - |
Rhodobacter capsulatus | |
0.87 | - |
reduced methyl viologen | - |
Rhodobacter capsulatus | |
13 | - |
nitrate | reaction with bromophenol blue | Rhodobacter capsulatus | |
80 | - |
ferricytochrome c | - |
Rhodobacter capsulatus | |
100 | - |
oxidized 2,6-dichlorophenolindophenol | - |
Rhodobacter capsulatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
1 * 46000 + 1 * 95000, SDS-PAGE | Rhodobacter capsulatus |
95000 | - |
1 * 46000 + 1 * 95000, SDS-PAGE | Rhodobacter capsulatus |
144000 | - |
non-denaturing pore gradient PAGE | Rhodobacter capsulatus |
147000 | - |
gel filtration | Rhodobacter capsulatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodobacter capsulatus | - |
E1F1 | - |
Rhodobacter capsulatus E1F1 | - |
E1F1 | - |
Purification (Comment) | Organism |
---|---|
- |
Rhodobacter capsulatus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rhodobacter capsulatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ferricytochrome c + NADH | - |
Rhodobacter capsulatus | 2 ferrocytochrome c + NAD+ + H+ | - |
? | |
2 ferricytochrome c + NADH | - |
Rhodobacter capsulatus E1F1 | 2 ferrocytochrome c + NAD+ + H+ | - |
? | |
nitrate + NADH | - |
Rhodobacter capsulatus | nitrite + NAD+ + H2O | - |
? | |
nitrate + NADH | - |
Rhodobacter capsulatus E1F1 | nitrite + NAD+ + H2O | - |
? | |
oxidized 2,6-dichlorophenolindophenol + NADH | - |
Rhodobacter capsulatus | ? + NAD+ | - |
? | |
oxidized 2,6-dichlorophenolindophenol + NADH | - |
Rhodobacter capsulatus E1F1 | ? + NAD+ | - |
? | |
oxidized 2,6-dichlorophenolindophenol + NADH + H+ | - |
Rhodobacter capsulatus | reduced 2,6-dichlorophenolindophenol + NAD+ | - |
r | |
reduced bromophenol blue + nitrate | - |
Rhodobacter capsulatus | bromophenol blue + nitrite | - |
? | |
reduced bromophenol blue + nitrate | - |
Rhodobacter capsulatus E1F1 | bromophenol blue + nitrite | - |
? | |
reduced methyl viologen + nitrate | - |
Rhodobacter capsulatus | methyl viologen + nitrite | - |
? | |
reduced methyl viologen + nitrate | - |
Rhodobacter capsulatus E1F1 | methyl viologen + nitrite | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 46000 + 1 * 95000, SDS-PAGE | Rhodobacter capsulatus |
More | diaphorase activity is located in the small subunit | Rhodobacter capsulatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
NADH-diaphorase activity | Rhodobacter capsulatus |
9 | - |
NADH-nitrate reductase activity | Rhodobacter capsulatus |
9.5 | - |
methyl viologen-nitrate reductase activity | Rhodobacter capsulatus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Rhodobacter capsulatus |