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Literature summary for 1.6.99.1 extracted from

  • Okamoto, N.; Yamaguchi, K.; Mizohata, E.; Tokuoka, K.; Uchiyama, N.; Sugiyama, S.; Matsumura, H.; Inaka, K.; Urade, Y.; Inoue, T.
    Structural insight into the stereoselective production of PGF2alpha by old yellow enzyme from Trypanosoma cruzi (2011), J. Biochem., 150, 563-568.
    View publication on PubMed
Search for more literature for 1.6.99.1

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of the enzyme in Escherichia coli Trypanosoma cruzi

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant enzyme in a ternary complex with inhibitor 12-oxophytodienoate and FMN, X-ray diffraction structure determination and analysis at 2.05 A resolution Trypanosoma cruzi

Inhibitors

Inhibitors Comment Organism Structure
(9R,13R)-12-oxophytodienoate
-
 Trypanosoma cruzi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Trypanosoma cruzi old yellow enzyme is an NADPH oxidoreductase capable of reducing a variety of compounds, it catalyzes one- or two-electron reduction ?
-
 ?
NADPH + H+ + acceptor Trypanosoma cruzi
-
 NADP+ + reduced acceptor
-
 ?
NADPH + H+ + prostaglandin H2 Trypanosoma cruzi via FMN cofactor, stereoselective reaction mechanism NADP+ + prostaglandin F2alpha
-
 ?

Organism

Organism UniProt Comment Textmining
Trypanosoma cruzi
-
-
-

Reaction

Reaction Comment Organism Reaction ID
NADPH + H+ + acceptor = NADP+ + reduced acceptor reaction mechanism involving a conserved triad of Glu193-His284-Arg249, overview Trypanosoma cruzi
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information old yellow enzyme is an NADPH oxidoreductase capable of reducing a variety of compounds, it catalyzes one- or two-electron reduction Trypanosoma cruzi ?
-
 ?
NADPH + H+ + 4-hydroxybenzaldehyde
-
 Trypanosoma cruzi NADP+ + ?
-
 ?
NADPH + H+ + acceptor
-
 Trypanosoma cruzi NADP+ + reduced acceptor
-
 ?
NADPH + H+ + prostaglandin H2 via FMN cofactor, stereoselective reaction mechanism Trypanosoma cruzi NADP+ + prostaglandin F2alpha
-
 ?

Synonyms

Synonyms Comment Organism
old yellow enzyme
-
 Trypanosoma cruzi
OYE
-
 Trypanosoma cruzi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Trypanosoma cruzi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Trypanosoma cruzi

Cofactor

Cofactor Comment Organism Structure
FMN prosthetic group Trypanosoma cruzi

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.057
-
 (9R,13R)-12-oxophytodienoate pH 7.0, 25°C Trypanosoma cruzi

General Information

General Information Comment Organism
additional information structure modelling of the enzyme with bound substrate prostaglandin H2 Trypanosoma cruzi