KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
N-ethylmaleimide | or below, pH 7.5, 25°C, GST-fusion protein | Bacillus subtilis | |
0.001 | - |
N-ethylmaleimide | or below, pH 7.5, 25°C, native protein | Bacillus subtilis | |
0.045 | - |
nitroglycerin | pH 7.5, 25°C, GST-fusion protein | Bacillus subtilis | |
0.109 | - |
nitroglycerin | pH 7.5, 25°C, native protein | Bacillus subtilis | |
0.123 | - |
2,4,6-trinitrotoluene | pH 7.5, 25°C, GST-fusion protein | Bacillus subtilis | |
0.186 | - |
cyclohex-2-enone | pH 7.5, 25°C, GST-fusion protein | Bacillus subtilis | |
0.293 | - |
cyclohex-2-enone | pH 7.5, 25°C, native protein | Bacillus subtilis | |
0.705 | - |
2,4,6-trinitrotoluene | pH 7.5, 25°C, native protein | Bacillus subtilis | |
1.853 | - |
trans-hex-2-enal | pH 7.5, 25°C, GST-fusion protein | Bacillus subtilis | |
2.602 | - |
trans-hex-2-enal | pH 7.5, 25°C, native protein | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37400 | - |
4 * 37400, SDS-PAGE, native protein, 4 * 62900, SDS-PAGE, GST-tagged protein, in 3:1 dimer/tetramer equilibrium | Bacillus subtilis |
38300 | - |
2 * 38300, SDs-PAGE, His-tagged protein, 2 * 62900, SDS-PAGE, GST-tagged protein, in 3:1 dimer/tetramer equilibrium | Bacillus subtilis |
62900 | - |
2 * 38300, SDs-PAGE, His-tagged protein, 2 * 62900, SDS-PAGE, GST-tagged protein, in 3:1 dimer/tetramer equilibrium | Bacillus subtilis |
62900 | - |
4 * 37400, SDS-PAGE, native protein, 4 * 62900, SDS-PAGE, GST-tagged protein, in 3:1 dimer/tetramer equilibrium | Bacillus subtilis |
76500 | - |
gel filtration, His-tagged protein | Bacillus subtilis |
114400 | - |
gel filtration, GST-tagged protein | Bacillus subtilis |
147000 | - |
gel filtration, native protein | Bacillus subtilis |
247500 | - |
gel filtration, GST-tagged protein | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
native enzyme and expression of N-terminal glutathione-S-transferase fusion and a C-terminal His-tagged form in Escherichia coli | - |
Purification (Comment) | Organism |
---|---|
N-terminal glutathione-S-transferase fusion and a C-terminal His-tagged form expressed in Escherichia coli | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADPH + H+ + 2,4,6-trinitrotoluene | - |
Bacillus subtilis | NADP+ + ? | - |
? | |
NADPH + H+ + cyclohex-2-enone | - |
Bacillus subtilis | NADP+ + ? | - |
? | |
NADPH + H+ + N-ethylmaleimide | - |
Bacillus subtilis | NADP+ + ? | - |
? | |
NADPH + H+ + nitroglycerin | - |
Bacillus subtilis | NADP+ + ? | - |
? | |
NADPH + H+ + trans-hex-2-enal | - |
Bacillus subtilis | NADP+ + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 38300, SDs-PAGE, His-tagged protein, 2 * 62900, SDS-PAGE, GST-tagged protein, in 3:1 dimer/tetramer equilibrium | Bacillus subtilis |
tetramer | 4 * 37400, SDS-PAGE, native protein, 4 * 62900, SDS-PAGE, GST-tagged protein, in 3:1 dimer/tetramer equilibrium | Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
YqjM | - |
Bacillus subtilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | half-reaction kinetics of tagged and untagged enzyme | Bacillus subtilis | |
0.53 | - |
2,4,6-trinitrotoluene | pH 7.5, 25°C, GST-fusion protein | Bacillus subtilis | |
0.75 | - |
N-ethylmaleimide | pH 7.5, 25°C, GST-fusion protein | Bacillus subtilis | |
0.82 | - |
nitroglycerin | pH 7.5, 25°C, GST-fusion protein | Bacillus subtilis | |
1.17 | - |
trans-hex-2-enal | pH 7.5, 25°C, GST-fusion protein | Bacillus subtilis | |
1.2 | - |
cyclohex-2-enone | pH 7.5, 25°C, GST-fusion protein | Bacillus subtilis | |
3.19 | - |
2,4,6-trinitrotoluene | pH 7.5, 25°C, native protein | Bacillus subtilis | |
3.72 | - |
nitroglycerin | pH 7.5, 25°C, native protein | Bacillus subtilis | |
4.38 | - |
cyclohex-2-enone | pH 7.5, 25°C, native protein | Bacillus subtilis | |
4.68 | - |
trans-hex-2-enal | pH 7.5, 25°C, native protein | Bacillus subtilis | |
8.07 | - |
N-ethylmaleimide | pH 7.5, 25°C, native protein | Bacillus subtilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Bacillus subtilis |