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Literature summary for 1.6.5.6 extracted from

  • Shimizu, S.; Hattori, S.; Hata, H.; Yamada, H.
    A novel fungal enzyme, NADPH-dependent carbonyl reductase, showing high specificity to conjugated polyketones (1988), Eur. J. Biochem., 174, 37-44.
    View publication on PubMed
Search for more literature for 1.6.5.6

Inhibitors

Inhibitors Comment Organism Structure
1,3-cyclopentadione 1.0 mM Mucor ambiguus
3-methyl-1,2-cyclohexadione 1.0 mM Mucor ambiguus
5,5'-dithiobis(2-nitrobenzoic acid) 0.1 mM Mucor ambiguus
8-hydroxyquinoline 0.1 mM Mucor ambiguus
CdCl2 1.0 mM Mucor ambiguus
cyclohexadione 1.0 mM Mucor ambiguus
dicoumarol 0.1 mM Mucor ambiguus
FeCl2 1.0 mM Mucor ambiguus
additional information overview Mucor ambiguus
NADP+ 1.0 mM Mucor ambiguus
o-phenanthroline 1.0 mM Mucor ambiguus
quercetin 0.01 mM Mucor ambiguus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0123
-
 1-methylisatin
-
 Mucor ambiguus
0.05
-
 isatin
-
 Mucor ambiguus
0.053
-
 beta-naphthoquinone
-
 Mucor ambiguus
0.135
-
 NADPH
-
 Mucor ambiguus
0.21
-
 acenaphthenequinone
-
 Mucor ambiguus
0.24
-
 dihydro-5-(2-pentyl)-4-methyl-4-propyl-2,3-furanedione
-
 Mucor ambiguus
0.38
-
 dihydro-5-(2-pentyl)-4,4-dimethyl-2,3-furanedione
-
 Mucor ambiguus
0.41
-
 p-toluquinone
-
 Mucor ambiguus
0.58
-
 dihydro-5-(3-pentyl)-4,4-dimethyl-2,3-furanedione
-
 Mucor ambiguus
0.71
-
 ketopantoyl lactone
-
 Mucor ambiguus
1.61
-
 dihydro-5-(2-butyl)-4-methyl-4-ethyl-2,3-furanedione
-
 Mucor ambiguus
2.5
-
 alloxan
-
 Mucor ambiguus
3.3
-
 dihydro-4-methyl-4-propyl-2,3-furanedione
-
 Mucor ambiguus
4.17
-
 dihydro-5-isopropyl-4,4-dimethyl-2,3-furanedione
-
 Mucor ambiguus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27500
-
 2 * 27500, SDS-PAGE Mucor ambiguus
44000
-
 high-performance gel-permeation liquid chromatography, TSK G-3000 SW Mucor ambiguus
54000
-
 gel filtration, Sephadex G-100 Mucor ambiguus

Organism

Organism UniProt Comment Textmining
Mucor ambiguus
-
-
-
Mucor ambiguus AKU 3006
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purification to apparent homogenity Mucor ambiguus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-methylisatin + NADPH
-
 Mucor ambiguus NADP+ + ?
-
 ir
5-bromoisatin + NADPH
-
 Mucor ambiguus NADP+ + ?
-
 ir
5-methylisatin + NADPH
-
 Mucor ambiguus NADP+ + ?
-
 ir
acenaphthenequinone + NADPH reduced velocity Mucor ambiguus NADP+ + acenaphthylene-1,2-diol
-
 ir
alloxan + NADPH
-
 Mucor ambiguus NADP+ + ?
-
 ir
alpha-naphthoquinone + NADPH
-
 Mucor ambiguus NADP+ + naphthalene-1,4-diol
-
 ir
alpha-naphthoquinone + NADPH
-
 Mucor ambiguus AKU 3006 NADP+ + naphthalene-1,4-diol
-
 ir
beta-naphthoquinone + NADPH
-
 Mucor ambiguus NADP+ + naphthalene-1,2-diol
-
 ir
beta-naphthoquinone + NADPH
-
 Mucor ambiguus AKU 3006 NADP+ + naphthalene-1,2-diol
-
 ir
dihydro-4-methyl-4-propyl-2,3-furanedione + NADPH
-
 Mucor ambiguus NADP+ + 4-methyl-4-propyl-4,5-dihydrofuran-2,3-diol
-
 ir
dihydro-5-(2-butyl)-4-methyl-4-ethyl-2,3-furanedione + NADPH reduced velocity Mucor ambiguus NADP+ + 5-sec-butyl-4-ethyl-4-methyl-4,5-dihydrofuran-2,3-diol
-
 ir
dihydro-5-(2-pentyl)-4,4-dimethyl-2,3-furanedione + NADPH
-
 Mucor ambiguus NADP+ + 4,4-dimethyl-5-(1-methylbutyl)-4,5-dihydrofuran-2,3-diol
-
 ir
dihydro-5-(2-pentyl)-4-methyl-4-propyl-2,3-furanedione + NADPH reduced velocity Mucor ambiguus NADP+ + 4-methyl-5-(1-methylbutyl)-4-propyl-4,5-dihydrofuran-2,3-diol
-
 ir
dihydro-5-(3-pentyl)-4,4-dimethyl-2,3-furanedione + NADPH
-
 Mucor ambiguus NADP+ + 5-(1-ethylpropyl)-4,4-dimethyl-4,5-dihydrofuran-2,3-diol
-
 ir
dihydro-5-isopropyl-4,4-dimethyl-2,3-furanedione + NADPH
-
 Mucor ambiguus NADP+ + 5-isopropyl-4,4-dimethyl-4,5-dihydrofuran-2,3-diol
-
 ir
isatin + NADPH
-
 Mucor ambiguus NADP+ + 1-methyl-1H-indole-2,3-diol
-
 ir
ketopantoyl lactone + NADPH
-
 Mucor ambiguus NADP+ + ?
-
 ir
ninhydrin + NADPH
-
 Mucor ambiguus NADP+ + ?
-
 ir
p-benzoquinone + NADPH
-
 Mucor ambiguus NADP+ + hydroquinone
-
 ir
p-benzoquinone + NADPH
-
 Mucor ambiguus AKU 3006 NADP+ + hydroquinone
-
 ir
p-toluquinone + NADPH + H+
-
 Mucor ambiguus NADP+ + 2-methylbenzene-1,4-diol
-
 ir
p-toluquinone + NADPH + H+
-
 Mucor ambiguus AKU 3006 NADP+ + 2-methylbenzene-1,4-diol
-
 ir
phenanthrenequinone + NADPH
-
 Mucor ambiguus NADP+ + ?
-
 ir
phenanthrenequinone + NADPH
-
 Mucor ambiguus AKU 3006 NADP+ + ?
-
 ir

Subunits

Subunits Comment Organism
dimer 2 * 27500, SDS-PAGE Mucor ambiguus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 reduction of ketopantoyl lactone Mucor ambiguus
45
-
 reduction of isatin Mucor ambiguus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15 50 for isatin and ketopantoyl lactone as substrates Mucor ambiguus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
 40 15% of control activity at 50°C Mucor ambiguus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7 for isatin reduction Mucor ambiguus
6
-
 for ketopantoyl lactone reduction Mucor ambiguus

pH Range

pH Minimum pH Maximum Comment Organism
5 9 with different buffers Mucor ambiguus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 7 91% of control activity at pH 9, 79% of control activity at pH 12, no activity at pH 4.0 Mucor ambiguus

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Mucor ambiguus