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Literature summary for 1.6.5.4 extracted from

  • Maynard, D.; Kumar, V.; Sproi, J.; Dietz, K.J.
    12-Oxophytodienoic acid reductase 3 (OPR3) functions as NADPH-dependent alpha,beta-ketoalkene reductase in detoxification and monodehydroascorbate reductase in redox homeostasis (2020), Plant Cell Physiol., 61, 584-595 .
    View publication on PubMed
Search for more literature for 1.6.5.4

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
 NADPH pH 7.8, temperature not specified in the publication Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9FUP0 cf. EC 1.3.1.42
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ferricyanide + NADPH + H+
-
 Arabidopsis thaliana 2 ferrocyanide + NADP+
-
 ?
monodehydroascorbate + NADPH + H+
-
 Arabidopsis thaliana ascorbate + NADP+
-
 ?

Subunits

Subunits Comment Organism
? x * 42000, SDS-PAGE, x * 42690, calculated from sequence Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
12-oxophytodienoate reductase 3
-
 Arabidopsis thaliana
OPR3
-
 Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
FMN absorption maxima at 280, 374 and 467 nm Arabidopsis thaliana

General Information

General Information Comment Organism
physiological function OPR3 is a bifunctional enzyme with NADPH-dependent alpha,beta-ketoalkene double-bond reductase and monodehydroascorbate reductase activities. OPR3 mutants show a slightly less-reduced ascorbate pool in leaves in line with the MDHAR activity of OPR3 in vitro. OPR3 catalyzes the reduction of a wide spectrum of electrophilic species that share a reactivity toward the glutathione and ascorbate. 12-oxophytodienoic acid reacts with ascorbate to form an ascorbate-12-oxophytodienoic acid adduct, but in addition OPR3 has the ability to regenerate ascorbate from monodehydroascorbate Arabidopsis thaliana