Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Hordeum vulgare |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
NADH | in the presence of hemoglobin | Hordeum vulgare | |
0.6 | - |
monodehydroascorbate | in the presence of hemoglobin | Hordeum vulgare |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Hordeum vulgare | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
SDS-PAGE | Hordeum vulgare |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hordeum vulgare | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Phenyl Superose gel filtration, Blue Sepharose column chromatography and DEAE cellulose column chromatography | Hordeum vulgare |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | - |
Hordeum vulgare | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
185 | - |
using ascorbate free radical as substrate | Hordeum vulgare |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADH + monodehydroascorbate | - |
Hordeum vulgare | NAD+ + ascorbate | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 40000, SDS-PAGE | Hordeum vulgare |
Synonyms | Comment | Organism |
---|---|---|
MDHAR | - |
Hordeum vulgare |