Literature summary for 1.6.5.4 extracted from

Sano, S.; Kang, Y.N.; Shigemizu, H.; Morishita, N.; Yoon, H.J.; Saito, K.; Asada, K.; Mikami, B.
Crystallization and preliminary crystallographic analysis of monodehydroascorbate radical reductase from cucumber (2004), Acta Crystallogr. Sect. D, 60, 1498-1499.

Cloned(Commentary)

Cloned (Comment)	Organism
overexpression in Escherichia coli	Cucumis sativus

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant enzyme, immediately after purification by hanging drop vapour diffusion method, 0.005 ml of protein solution containing 10 mg/ml protein, mixed with equal volume of reservoir solution containing 20-25% PEG 6000, 0.1 M CaCl2, 50 mM Tris-HCl, pH 8.0, equilibration against 1 ml reservoir solution, 20°C, 1 week, cryoprotection by 30% glycerol, X-ray diffraction structure determination and analysis at 2.4 A resolution	Cucumis sativus

Crystallization (Comment)

Organism

purified recombinant enzyme, immediately after purification by hanging drop vapour diffusion method, 0.005 ml of protein solution containing 10 mg/ml protein, mixed with equal volume of reservoir solution containing 20-25% PEG 6000, 0.1 M CaCl2, 50 mM Tris-HCl, pH 8.0, equilibration against 1 ml reservoir solution, 20°C, 1 week, cryoprotection by 30% glycerol, X-ray diffraction structure determination and analysis at 2.4 A resolution

Cucumis sativus

Inhibitors

Inhibitors	Comment	Organism	Structure
thiol-modifiying reagents	inhibition at the step of FAD reduction by NADH producing a charge-transfer complex	Cucumis sativus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	kinetics	Cucumis sativus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
47300	-	x * 47300, SDS-PAGE	Cucumis sativus

Organism

Organism	UniProt	Comment	Textmining
Cucumis sativus	-	cucumber	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant from Escherichia coli	Cucumis sativus

Reaction

Reaction	Comment	Organism	Reaction ID
NADH + H+ + 2 monodehydroascorbate = NAD+ + 2 ascorbate	ping-pong mechanism	Cucumis sativus	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
NAD(P)H + monodehydroascorbate	reduction of the radical monodehydroascorbate, via FAD as intermediary electron acceptor, FAD reduction by NADH producing a charge-transfer complex	Cucumis sativus	NAD(P)+ + ascorbate	-	?

Subunits

Subunits	Comment	Organism
?	x * 47300, SDS-PAGE	Cucumis sativus

Synonyms

Synonyms	Comment	Organism
MDA	-	Cucumis sativus
monodehydroascorbate radical reductase	-	Cucumis sativus

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Cucumis sativus
NAD(P)H	-	Cucumis sativus

pI Value

Organism	Comment	pI Value Maximum	pI Value
Cucumis sativus	sequence calculation	-	6