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Literature summary for 1.6.5.4 extracted from
- Purification and properties of ascorbate free-radical reductase from potato tubers (1986), Planta, 167, 521-526.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-[[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]-amino]ethanesulfonic acid | - |
Solanum tuberosum |  |
| Imidazole buffer | - |
Solanum tuberosum | |
| Mersalyl | - |
Solanum tuberosum | |
| NEM | - |
Solanum tuberosum | |
| PCMB | - |
Solanum tuberosum | |
| phosphate | - |
Solanum tuberosum | |
| Tris | - |
Solanum tuberosum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0077 | - |
NADH | - |
Solanum tuberosum | |
| 0.03 | - |
NADPH | - |
Solanum tuberosum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 41500 | - |
x * 41500, SDS-PAGE | Solanum tuberosum |
| 42000 | - |
gel filtration | Solanum tuberosum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Solanum tuberosum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Solanum tuberosum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| tuber | - |
Solanum tuberosum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 61 | - |
- |
Solanum tuberosum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, pH 8, 30 d, almost complete loss of activity, residual activity up to 38% when stored in presence of 1-10 mM MgSO4 | Solanum tuberosum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| NADH + monodehydroascorbate | - |
Solanum tuberosum | NAD+ + ascorbate | - |
? | |
| NADPH + monodehydroascorbate | - |
Solanum tuberosum | NADP+ + ascorbate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | x * 41500, SDS-PAGE | Solanum tuberosum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
1 min, 20% loss of activity | Solanum tuberosum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Solanum tuberosum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | - |
Solanum tuberosum | |
| NADPH | - |
Solanum tuberosum | |
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Release 2023.1 (January 2023)
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