Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | about 10fold induction of enzyme expression by H2O2 | Pseudomonas putida |
Cloned (Comment) | Organism |
---|---|
gene chrR, quantitative expression analysis and profile | Pseudomonas putida |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of ChrR overexpressing and knockout mutant strains showing increased and abolished tolerance of H2O2, respectively, enhancing the activity of ChrR in a chromate-remediating bacterial strain may not only increase the rate of chromate transformation, it may also augment the capacity of these cells to withstand the unavoidable production of H2O2 that accompanies chromate reduction, overview | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetic analysis of the quinone reductase activity | Pseudomonas putida | |
0.11 | - |
benzoquinone | pH 7.5, 70°C | Pseudomonas putida | |
0.12 | - |
coenzyme Q1 | pH 8.5, 60°C | Pseudomonas putida | |
0.21 | - |
duroquinone | pH 8.0, 70°C | Pseudomonas putida | |
0.23 | - |
menadione | pH 8.0, 60°C | Pseudomonas putida | |
0.26 | - |
chromate(IV) | pH 5.0, 70°C | Pseudomonas putida |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Pseudomonas putida | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pseudomonas putida | ChrR minimizes intracellular H2O2 stress | ? | - |
? | |
additional information | Pseudomonas putida KT 2240 | ChrR minimizes intracellular H2O2 stress | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
gene chrR | - |
Pseudomonas putida KT 2240 | - |
gene chrR | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
NAD(P)H + H+ + a quinone = NAD(P)+ + a hydroquinone | reaction mechanism | Pseudomonas putida |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | H2O2-scavenging and growth of wild-type, overexpressing, and knockout cells, overview | Pseudomonas putida | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzoquinone + NADH | - |
Pseudomonas putida | ? | - |
? | |
benzoquinone + NADH | - |
Pseudomonas putida KT 2240 | ? | - |
? | |
chromate(IV) + H2O | - |
Pseudomonas putida | chromate(III) + H2O2 | - |
? | |
chromate(IV) + H2O | - |
Pseudomonas putida KT 2240 | chromate(III) + H2O2 | - |
? | |
coenzyme Q1 + NADH | - |
Pseudomonas putida | ? | - |
? | |
duroquinone + NADH | - |
Pseudomonas putida | ? | - |
? | |
menadione + NADH | - |
Pseudomonas putida | ? | - |
? | |
menadione + NADH | - |
Pseudomonas putida KT 2240 | ? | - |
? | |
additional information | ChrR minimizes intracellular H2O2 stress | Pseudomonas putida | ? | - |
? | |
additional information | ChrR reduces quinones by simultaneous two-electron transfer, avoiding formation of highly reactive semiquinone intermediates and producing quinols that promote tolerance of H2O2 | Pseudomonas putida | ? | - |
? | |
additional information | ChrR minimizes intracellular H2O2 stress | Pseudomonas putida KT 2240 | ? | - |
? | |
additional information | ChrR reduces quinones by simultaneous two-electron transfer, avoiding formation of highly reactive semiquinone intermediates and producing quinols that promote tolerance of H2O2 | Pseudomonas putida KT 2240 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
chromate reductase | - |
Pseudomonas putida |
ChrR | - |
Pseudomonas putida |
Quinone reductase | - |
Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 70 | - |
Pseudomonas putida |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.8 | - |
chromate(IV) | pH 5.0, 70°C | Pseudomonas putida | |
6.4 | - |
coenzyme Q1 | pH 8.5, 60°C | Pseudomonas putida | |
25 | - |
duroquinone | pH 8.0, 70°C | Pseudomonas putida | |
440 | - |
menadione | pH 8.0, 60°C | Pseudomonas putida | |
930 | - |
benzoquinone | pH 7.5, 70°C | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
chromate reductase activity | Pseudomonas putida |
7.5 | 8.5 | quinone reductase activity | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
flavin | flavoenzyme | Pseudomonas putida | |
NADH | - |
Pseudomonas putida |