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Literature summary for 1.6.5.2 extracted from

  • Wu, K.; Knox, R.; Sun, X.Z.; Joseph, P.; Jaiswal, A.K.; Zhang, D.; Deng, P.S.K.; Chen, S.
    Catalytic properties of NAD(P)H:quinone oxidoreductase-2 (NQO2), a dihydronicotinamide riboside dependent oxidoreductase (1997), Arch. Biochem. Biophys., 347, 221-228.
    View publication on PubMed
Search for more literature for 1.6.5.2

Cloned(Commentary)

Cloned (Comment) Organism
NAD(P)H:quinone acceptor oxidoreductase 2 Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
3,3'-methylene-bis(4-hydroxycoumarin) 0.01 mM, 25% inhibition, recombinant NAD(P)H:quinone acceptor oxidoreductase 2 Homo sapiens
4',5,7-trihydroxyflavone 0.01 mM, 61% inhibition, recombinant NAD(P)H:quinone acceptor oxidoreductase 2 Homo sapiens
alpha-naphthoflavone 0.01 mM, 75% inhibition, recombinant NAD(P)H:quinone acceptor oxidoreductase 2 Homo sapiens
beta-naphthoflavone 0.01 mM, 76% inhibition, recombinant NAD(P)H:quinone acceptor oxidoreductase 2 Homo sapiens
chrysin 0.01 mM, 100% inhibition, recombinant NAD(P)H:quinone acceptor oxidoreductase 2 Homo sapiens
galangin 0.01 mM, 100% inhibition, recombinant NAD(P)H:quinone acceptor oxidoreductase 2 Homo sapiens
morin 0.01 mM, 95% inhibition, recombinant NAD(P)H:quinone acceptor oxidoreductase 2 Homo sapiens
quercetin 0.01 mM, 100% inhibition, recombinant NAD(P)H:quinone acceptor oxidoreductase 2 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0023
-
 menadione recombinant NAD(P)H:quinone acceptor oxidoreductase 2 Homo sapiens
0.0027
-
 menadione recombinant NAD(P)H:quinone acceptor oxidoreductase 1 Homo sapiens
0.0047
-
 Methyl red recombinant NAD(P)H:quinone acceptor oxidoreductase 2 Homo sapiens
0.007
-
 oxidized 2,6-dichloroindophenol recombinant NAD(P)H:quinone acceptor oxidoreductase 2 Homo sapiens
0.012
-
 oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium recombinant NAD(P)H:quinone acceptor oxidoreductase 2 Homo sapiens
0.018
-
 oxidized 2,6-dichloroindophenol recombinant NAD(P)H:quinone acceptor oxidoreductase 1 Homo sapiens
0.028
-
 reduced dihydronicotinamide riboside recombinant NAD(P)H:quinone acceptor oxidoreductase 2 Homo sapiens
0.028
-
 Methyl red recombinant NAD(P)H:quinone acceptor oxidoreductase 1 Homo sapiens
0.07
-
 reduced dihydronicotinamide riboside recombinant NAD(P)H:quinone acceptor oxidoreductase 1 Homo sapiens
0.26
-
 5-(aziridin-1-yl)-2,4-dinitrobenzamide recombinant NAD(P)H:quinone acceptor oxidoreductase 2 Homo sapiens
1.37
-
 5-(aziridin-1-yl)-2,4-dinitrobenzamide recombinant NAD(P)H:quinone acceptor oxidoreductase 2 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
 2 * 26000, NAD(P)H:quinone acceptor oxidoreductase 2, SDS-PAGE Homo sapiens
50000
-
 NAD(P)H:quinone acceptor oxidoreductase 2, gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 NAD(P)H:quinone acceptor oxidoreductase 2
-

Purification (Commentary)

Purification (Comment) Organism
recombinant NAD(P)H:quinone acceptor oxidoreductase 2, affinity chromatography on Affi-gel blue Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methyl red + NAD(P)H
-
 Homo sapiens reduced methyl red + NAD(P)+
-
 ?
oxidized 2,6-dichlorophenolindophenol + NADH + H+
-
 Homo sapiens reduced 2,6-dichlorophenolindophenol + NAD+
-
 r
oxidized 2,6-dichlorophenolindophenol + NADPH + H+
-
 Homo sapiens reduced 2,6-dichlorophenolindophenol + NADP+
-
 r
oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium + NADPH + H+
-
 Homo sapiens reduced 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium + NADP+
-
 r
oxidized methyl red + reduced dihydronicotinamide riboside + H+
-
 Homo sapiens reduced methyl red + oxidized dihydronicotinamide riboside
-
 r

Subunits

Subunits Comment Organism
dimer 2 * 26000, NAD(P)H:quinone acceptor oxidoreductase 2, SDS-PAGE Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.01
-
 5-(aziridin-1-yl)-2,4-dinitrobenzamide recombinant NAD(P)H:quinone acceptor oxidoreductase 2 Homo sapiens
0.333
-
 Methyl red recombinant NAD(P)H:quinone acceptor oxidoreductase 1 Homo sapiens
3.83
-
 Methyl red recombinant NAD(P)H:quinone acceptor oxidoreductase 2 Homo sapiens
6
-
 5-(aziridin-1-yl)-2,4-dinitrobenzamide recombinant NAD(P)H:quinone acceptor oxidoreductase 2 Homo sapiens
11
-
 oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium recombinant NAD(P)H:quinone acceptor oxidoreductase 2 Homo sapiens
38.3
-
 menadione recombinant NAD(P)H:quinone acceptor oxidoreductase 2 Homo sapiens
43.3
-
 reduced dihydronicotinamide riboside recombinant NAD(P)H:quinone acceptor oxidoreductase 2 Homo sapiens
46.7
-
 oxidized 2,6-dichlorophenolindophenol recombinant NAD(P)H:quinone acceptor oxidoreductase 2 Homo sapiens
517
-
 reduced dihydronicotinamide riboside recombinant NAD(P)H:quinone acceptor oxidoreductase 1 Homo sapiens
550
-
 menadione recombinant NAD(P)H:quinone acceptor oxidoreductase 1 Homo sapiens
600
-
 oxidized 2,6-dichlorophenolindophenol recombinant NAD(P)H:quinone acceptor oxidoreductase 1 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
FAD NAD(P)H:quinone acceptor oxidoreductase 2, contains 1 FAD per subunit Homo sapiens
nicotinamide riboside recombinant NAD(P)H:quinone acceptor oxidoreductase 2 Homo sapiens