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Literature summary for 1.6.5.2 extracted from
- Purification and characterization of two isofunctional forms of NAD(P)H: quinone reductase from mouse liver (1986), J. Biol. Chem., 261, 1372-1378.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hydrophilic isoform in the presence of FAD, hanging drop diffusion, 10 mg/ml enzyme, in 200 mM potassium phosphate, 0.5 mM EDTA, 0.5 mM, FAD, pH 8.0, 45-50% saturation of ammonium sulfate | Mus musculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3,3'-methylene-bis(4-hydroxycoumarin) | - |
Mus musculus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0000017 | - |
FAD | hydrophobic isoform | Mus musculus | |
| 0.0000027 | - |
FAD | hydrophilic isoform | Mus musculus | |
| 0.00196 | - |
menadione | hydrophilic isoform | Mus musculus | |
| 0.00247 | - |
menadione | hydrophobic isoform | Mus musculus | |
| 0.0581 | - |
NADPH | hydrophobic isoform | Mus musculus | |
| 0.0643 | - |
NADH | hydrophilic isoform | Mus musculus | |
| 0.066 | - |
NADPH | hydrophilic isoform | Mus musculus | |
| 0.0881 | - |
NADH | hydrophobic isoform | Mus musculus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Mus musculus | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
2 * 30000, hydrophilic and hydrophobic isoform, SDS-PAGE | Mus musculus |
| 52000 | - |
hydrophilic and hydrophobic isoforms, gel filtration | Mus musculus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
hydrophilic and hydrophobic isoform | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| affinity chromatography on Cibacron blue-Agarose | Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Mus musculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1720 | - |
hydrophilic isoform | Mus musculus |
| 1770 | - |
hydrophobic isoform | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,4-naphthoquinone + NAD(P)H | - |
Mus musculus | 1,4-naphthohydroquinone + NAD(P)+ | - |
? | |
| 2-methyl-1,4-naphthoquinone + NAD(P)H | artificial acceptor, trivial name menadione | Mus musculus | 2-methyl-1,4-naphthoquinol + NAD(P)+ | - |
? | |
| benzoquinone + 2 NAD(P)H + H+ | - |
Mus musculus | benzohydroquinone + 2 NAD(P)+ | - |
? | |
| menadione + NADH + H+ | - |
Mus musculus | menadiol + NAD+ | - |
r | |
| NAD(P)H + H+ + oxidized 2,6-dichlorophenolindophenol | - |
Mus musculus | NAD(P)+ + reduced 2,6-dichlorophenolindophenol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 30000, hydrophilic and hydrophobic isoform, SDS-PAGE | Mus musculus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000131 | - |
dicoumarol | hydrophilic isoform | Mus musculus | |
| 0.000288 | - |
dicoumarol | hydrophobic isoform | Mus musculus | |
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