Literature summary for 1.6.3.5 extracted from

Morrison, C.; Paskaleva, E.; Rios, M.; Beusse, T.; Blair, E.; Lin, L.; Hu, J.; Gorby, A.; Dodds, D.; Armiger, W.; Dordick, J.; Koffas, M.
Improved soluble expression and use of recombinant human renalase (2020), PLoS ONE, 15, e0242109 .

Search for more literature for 1.6.3.5

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
3D molecular models for mutants m5Ren1 and m6Ren1. Regions on the surface of the enzyme that are significantly changed include Q3R, C47R, R75S, S217D, and T329Q. Each of these mutations contributes to changes in polarity, charge, and hydrophilicity. Relative to wild-type, m6Ren1 exhibits a greater density of positively-charged residues around the active site	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
additional information	mutation of Ren1 to address poor solubility, low expression levels, misfolding, aggregation, and other stability factors. m5Ren1 introduces 19 mutations and m6Ren1 introduces 27 mutations to the primary sequence of renalase. Both m5Ren1 and m6Ren1 include one replacement for a cysteine residue to the wild-type sequence	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q5VYX0	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
19.8	-	MBP-tagged wild-type, pH not specified in the publication, temperature not specified in the publication	Homo sapiens
63	-	SUMO-tagged wild-type, pH not specified in the publication, temperature not specified in the publication	Homo sapiens
87.6	-	wild-type, pH not specified in the publication, temperature not specified in the publication	Homo sapiens
91	-	MBP-tagged mutant m5Ren1, pH not specified in the publication, temperature not specified in the publication	Homo sapiens
111	-	MBP-tagged mutant m6Ren1, pH not specified in the publication, temperature not specified in the publication	Homo sapiens
152	-	SUMO-tagged mutant m6Ren1, pH not specified in the publication, temperature not specified in the publication	Homo sapiens
289	-	SUMO-tagged mutant m5Ren1, pH not specified in the publication, temperature not specified in the publication	Homo sapiens
760	-	mutant m6Ren1, pH not specified in the publication, temperature not specified in the publication	Homo sapiens
1447	-	mutant m5Ren1, pH not specified in the publication, temperature not specified in the publication	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1,2-dihydro-beta-NADH + H+ + O2	-	Homo sapiens	beta-NAD+ + H2O2	-	?
1,6-dihydro-beta-NADH + H+ + O2	-	Homo sapiens	beta-NAD+ + H2O2	-	?

Synonyms

Synonyms	Comment	Organism
Ren1	-	Homo sapiens

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Release 2023.1 (January 2023)