Literature summary for 1.6.3.5 extracted from

Milani, M.; Ciriello, F.; Baroni, S.; Pandini, V.; Canevari, G.; Bolognesi, M.; Aliverti, A.
FAD-binding site and NADP reactivity in human renalase: a new enzyme involved in blood pressure regulation (2011), J. Mol. Biol., 411, 463-473.

Crystallization (Commentary)

Crystallization (Comment)	Organism
to 2.5 A resolution. Renalase adopts the p-hydroxybenzoate hydroxylase fold topology, comprising a Rossmann-fold-based flavin adenine dinucleotide-binding domain and a putative substrate-binding domain, the latter of which contains a five-stranded anti-parallel beta-sheet. A large cavity, facing the flavin ring, presumably represents the active site. The renalase active site is fully solvent exposed and lacks an aromatic cage for binding the substrate amino group	Homo sapiens

Crystallization (Comment)

Organism

to 2.5 A resolution. Renalase adopts the p-hydroxybenzoate hydroxylase fold topology, comprising a Rossmann-fold-based flavin adenine dinucleotide-binding domain and a putative substrate-binding domain, the latter of which contains a five-stranded anti-parallel beta-sheet. A large cavity, facing the flavin ring, presumably represents the active site. The renalase active site is fully solvent exposed and lacks an aromatic cage for binding the substrate amino group

Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q5VYX0	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	renalase has an extremely low diaphorase activity, displaying lower kcat but higher kcat/Km for NADH compared to NADPH	Homo sapiens	?	-	?

Cofactor

Cofactor	Comment	Organism	Structure
FAD	the FAD prosthetic group becomes slowly reduced when the enzyme is incubated with NADPH under anaerobiosis	Homo sapiens
additional information	a recognizable NADP-binding site is absent in the protein structure, enzyme shows poor affinity for, and poor reactivity towards, NADH and NADPH with Kd values of ca 2 mM	Homo sapiens

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Release 2023.1 (January 2023)