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Literature summary for 1.6.3.2 extracted from

  • Brown, D.M.; Upcroft, J.A.; Upcroft, P.
    A H2O-producing NADH oxidase from the protozoan parasite Giardia duodenalis (1996), Eur. J. Biochem., 241, 155-161.
    View publication on PubMed
Search for more literature for 1.6.3.2

Inhibitors

Inhibitors Comment Organism Structure
CuSO4 0.2 mM, 97% inhibition Giardia intestinalis
H2O2 0.5 mM, 50% inhibition Giardia intestinalis
HgCl2 0.5 mM, 80% inhibition Giardia intestinalis
additional information no inhibition by EDTA Giardia intestinalis
p-chloromercuribenzoate 0.05 mM, complete inhibition Giardia intestinalis
Quinacrine 1 mM, 73% inhibition Giardia intestinalis
Quinine 0.2 mM, 50% inhibition Giardia intestinalis
ZnSO4 0.1 mM, 98% inhibition Giardia intestinalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0042
-
 NADH pH 7.8, 22°C Giardia intestinalis
0.016
-
 NADPH pH 7.8, 22°C Giardia intestinalis

Metals/Ions

Metals/Ions Comment Organism Structure
additional information activity is not enhanced by the addition of various heavy metals which suggests that the enzyme does not contain any metal ions as co-factors Giardia intestinalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45116
-
 1 * 45116, calculated from sequence Giardia intestinalis
46000
-
 1 * 46000, SDS-PAGE Giardia intestinalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 NADPH + 2 H+ + O2 Giardia intestinalis the enzyme may be involved in maintenance of an optimum intracellular redox ratio 2 NADP+ + 2 H2O
-
 ?
2 NADPH + 2 H+ + O2 Giardia intestinalis BRIS/83/HEPU/106 the enzyme may be involved in maintenance of an optimum intracellular redox ratio 2 NADP+ + 2 H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Giardia intestinalis Q7M4I9
-
-
Giardia intestinalis BRIS/83/HEPU/106 Q7M4I9
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Giardia intestinalis

Source Tissue

Source Tissue Comment Organism Textmining
trophozoite
-
 Giardia intestinalis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 NADH + H+ + O2 NADPH is more efficient as electron donor compared to NADH Giardia intestinalis NAD+ + 2 H2O
-
 ?
2 NADH + H+ + O2 NADPH is more efficient as electron donor compared to NADH Giardia intestinalis BRIS/83/HEPU/106 NAD+ + 2 H2O
-
 ?
2 NADPH + 2 H+ + O2 the enzyme may be involved in maintenance of an optimum intracellular redox ratio Giardia intestinalis 2 NADP+ + 2 H2O
-
 ?
2 NADPH + 2 H+ + O2 O2 is the most effective electron acceptor, NADH or NADPH are not oxidized anaerobically Giardia intestinalis 2 NADP+ + 2 H2O
-
 ?
2 NADPH + 2 H+ + O2 the enzyme may be involved in maintenance of an optimum intracellular redox ratio Giardia intestinalis BRIS/83/HEPU/106 2 NADP+ + 2 H2O
-
 ?
2 NADPH + 2 H+ + O2 O2 is the most effective electron acceptor, NADH or NADPH are not oxidized anaerobically Giardia intestinalis BRIS/83/HEPU/106 2 NADP+ + 2 H2O
-
 ?
additional information cytochrome c is not reduced under any assay conditions used Giardia intestinalis ?
-
 ?
additional information cytochrome c is not reduced under any assay conditions used Giardia intestinalis BRIS/83/HEPU/106 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 46000, SDS-PAGE Giardia intestinalis
monomer 1 * 45116, calculated from sequence Giardia intestinalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
 Giardia intestinalis

pH Range

pH Minimum pH Maximum Comment Organism
7 9 active over the range pH 7-9 Giardia intestinalis

Cofactor

Cofactor Comment Organism Structure
FAD FAD is noncoveltly bound, 1 mol of FAD per mol of polypeptide Giardia intestinalis
NADH NADPH is more efficient as electron donor compared to NADH Giardia intestinalis
NADPH NADPH is more efficient as electron donor compared to NADH Giardia intestinalis

General Information

General Information Comment Organism
physiological function the enzyme may be involved in maintenance of an optimum intracellular redox ratio Giardia intestinalis