Cloned (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
General Stability | Organism |
---|---|
presence of trypsin at a 3:1 weight ratio | Saccharolobus solfataricus |
resistance to trypsin, cleavage of in two major fragments of about 21 and 14 kDa is obtained after incubation of the protein for 20 h at 37°C in the | Saccharolobus solfataricus |
the enzyme is resistant against chemical and physical denaturating agents | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.413 | - |
NADH | pH 5.5, 65°C | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36508 | - |
2 * 36508, calculated from sequence | Saccharolobus solfataricus |
38000 | - |
2 * 38000, SDS-PAGE | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Saccharolobus solfataricus MT-4 / DSM 5833 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.8 | - |
pH 5.5, 65°C | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADH + H+ + O2 | oxidizes NADH and, less efficiently, NADPH | Saccharolobus solfataricus | NAD+ + H2O2 | - |
? | |
NADH + H+ + O2 | oxidizes NADH and, less efficiently, NADPH | Saccharolobus solfataricus MT-4 / DSM 5833 | NAD+ + H2O2 | - |
? | |
NADPH + H+ + O2 | oxidizes NADH and, less efficiently, NADPH (2fold lower activity) | Saccharolobus solfataricus | NADP+ + H2O2 | - |
? | |
NADPH + H+ + O2 | oxidizes NADH and, less efficiently, NADPH (2fold lower activity) | Saccharolobus solfataricus MT-4 / DSM 5833 | NADP+ + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 38000, SDS-PAGE | Saccharolobus solfataricus |
homodimer | 2 * 36508, calculated from sequence | Saccharolobus solfataricus |
Synonyms | Comment | Organism |
---|---|---|
SsNOX38 | - |
Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
assay at | Saccharolobus solfataricus |
87 | - |
- |
Saccharolobus solfataricus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | 95 | 80°C: about 45% of maximal activity, 95°C: about 45% of maximal activity | Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
temperature for half-denaturation in presence of 8 M urea | Saccharolobus solfataricus |
87 | - |
the enzyme remains stable for 60 min | Saccharolobus solfataricus |
93 | - |
temperature for half-denaturation in absence of 8 M urea | Saccharolobus solfataricus |
105 | - |
t1/2: about 2 min | Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.4 | - |
NADH | pH 5.5, 65°C | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
assay at | Saccharolobus solfataricus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | contains 1 FAD molecule/subunit, the enzyme requires the presence of FAD as cofactor | Saccharolobus solfataricus | |
NADH | oxidizes NADH and, less efficiently, NADPH | Saccharolobus solfataricus | |
NADPH | 2fold lower activity compared to NADH | Saccharolobus solfataricus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10.7 | - |
NADH | pH 5.5, 65°C | Saccharolobus solfataricus |