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Literature summary for 1.6.2.6 extracted from

  • Saari, L.L.; Klucas, R.V.
    Ferric leghemoglobin reductase from soybean root nodules (1984), Arch. Biochem. Biophys., 231, 102-113.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
acetate slight Glycine max
iodoacetamide
-
Glycine max
nicotinate
-
Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0095
-
ferrileghemoglobin
-
Glycine max
0.0188
-
NADH
-
Glycine max

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Glycine max 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
2 * 54000, SDS-PAGE Glycine max
83000
-
equilibrium ultracentrifugation Glycine max
100000
-
gel filtration Glycine max

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NAD(P)H + ferrileghemoglobin Glycine max
-
NAD(P)+ + ferroleghemoglobin
-
?

Organism

Organism UniProt Comment Textmining
Glycine max
-
soybean
-

Purification (Commentary)

Purification (Comment) Organism
-
Glycine max

Source Tissue

Source Tissue Comment Organism Textmining
root nodule
-
Glycine max
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.218
-
reduction of ferrileghemoglobin Glycine max

Storage Stability

Storage Stability Organism
0°C, 37 days, 96% activity retains Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ferricytochrome c + NAD(P)H reduced at 53% of leghemoglobin rate Glycine max 2 ferrocytochrome c + NAD(P)+ + H+
-
?
NAD(P)H + ferrileghemoglobin
-
Glycine max NAD(P)+ + ferroleghemoglobin
-
?
NAD(P)H + ferrileghemoglobin 31% activity with NADPH Glycine max NAD(P)+ + ferroleghemoglobin
-
?
NAD(P)H + ferrimyoglobin
-
Glycine max NAD(P)+ + ferromyoglobin
-
?

Subunits

Subunits Comment Organism
dimer 2 * 54000, SDS-PAGE Glycine max

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
heat labile Glycine max

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.2
-
decrease of activity from 100% at pH 5.2, to 30% at pH 6.7, no measurements below 5.2 Glycine max

Cofactor

Cofactor Comment Organism Structure
NADH
-
Glycine max
NADPH 31% of NADH activity Glycine max