Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.6.2.4 extracted from

  • Gray, J.P.; Mishin, V.; Heck, D.E.; Laskin, D.L.; Laskin, J.D.
    Inhibition of NADPH cytochrome P450 reductase by the model sulfur mustard vesicant 2-chloroethyl ethyl sulfide is associated with increased production of reactive oxygen species (2010), Toxicol. Appl. Pharmacol., 247, 76-82.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information in contrast to the inhibitory effects of 2-chloroethyl ethyl sulfide on redox cycling b cytochrome P450 reductase, spontaneous hydrogen peroxide production by the enzyme is stimulated by 2-chloroethyl ethyl sulfide. This response is concentration-dependent in the range of 0.1-10 mM, reaching a maximum at 0.3 mM Mus musculus
additional information in contrast to the inhibitory effects of 2-chloroethyl ethyl sulfide on redox cycling b cytochrome P450 reductase, spontaneous hydrogen peroxide production by the enzyme is stimulated by 2-chloroethyl ethyl sulfide. This response is concentration-dependent in the range of 0.1-10 mM, reaching a maximum at 0.3 mM Homo sapiens
additional information in contrast to the inhibitory effects of 2-chloroethyl ethyl sulfide on redox cycling b cytochrome P450 reductase, spontaneous hydrogen peroxide production by the enzyme is stimulated by 2-chloroethyl ethyl sulfide. This response is concentration-dependent in the range of 0.1-10 mM, reaching a maximum at 0.3 mM Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
2-chloroethyl ethyl sulfide potent inhibitor Homo sapiens
2-chloroethyl ethyl sulfide potent inhibitor Mus musculus
2-chloroethyl ethyl sulfide potent inhibitor Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Homo sapiens
-
-
microsome
-
Rattus norvegicus
-
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Mus musculus
-
-
-
Rattus norvegicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-
MLE-15 cell type II lung epithelial cell Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ferricytochrome c + NADPH
-
Mus musculus 2 ferrocytochrome c + NADP+ + H+
-
?
2 ferricytochrome c + NADPH
-
Homo sapiens 2 ferrocytochrome c + NADP+ + H+
-
?
2 ferricytochrome c + NADPH
-
Rattus norvegicus 2 ferrocytochrome c + NADP+ + H+
-
?
NADPH + H+ + oxidized 2,6-dichlorophenolindophenol
-
Mus musculus NADP+ + reduced 2,6-dichlorophenolindophenol
-
?
NADPH + H+ + oxidized 2,6-dichlorophenolindophenol
-
Homo sapiens NADP+ + reduced 2,6-dichlorophenolindophenol
-
?
NADPH + H+ + oxidized 2,6-dichlorophenolindophenol
-
Rattus norvegicus NADP+ + reduced 2,6-dichlorophenolindophenol
-
?
NADPH + H+ + oxidized menadione
-
Mus musculus NADP+ + reduced menadione
-
?
NADPH + H+ + oxidized menadione
-
Homo sapiens NADP+ + reduced menadione
-
?
NADPH + H+ + oxidized menadione
-
Rattus norvegicus NADP+ + reduced menadione
-
?

Synonyms

Synonyms Comment Organism
cytochrome P450 reductase
-
Mus musculus
cytochrome P450 reductase
-
Homo sapiens
cytochrome P450 reductase
-
Rattus norvegicus
NADPH cytochrome P450 reductase
-
Mus musculus
NADPH cytochrome P450 reductase
-
Homo sapiens
NADPH cytochrome P450 reductase
-
Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
FAD
-
Mus musculus
FAD
-
Homo sapiens
FAD
-
Rattus norvegicus
FMN
-
Mus musculus
FMN
-
Homo sapiens
FMN
-
Rattus norvegicus
NADPH
-
Mus musculus
NADPH
-
Homo sapiens
NADPH
-
Rattus norvegicus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.38
-
using ferricytochrome c as cosubstrate, in 10 mM phosphate buffer (pH 7.4), at 37°C Homo sapiens 2-chloroethyl ethyl sulfide
1.9
-
using ferricytochrome c as cosubstrate, in 10 mM phosphate buffer (pH 7.4), at 37°C Mus musculus 2-chloroethyl ethyl sulfide
2.9
-
in 10 mM phosphate buffer (pH 7.4), at 37°C Rattus norvegicus 2-chloroethyl ethyl sulfide
3.3
-
using oxidized 2,6-dichlorophenolindophenol as cosubstrate, in 10 mM phosphate buffer (pH 7.4), at 37°C Mus musculus 2-chloroethyl ethyl sulfide
8.6
-
using oxidized 2,6-dichlorophenolindophenol as cosubstrate, in 10 mM phosphate buffer (pH 7.4), at 37°C Homo sapiens 2-chloroethyl ethyl sulfide