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Literature summary for 1.6.2.4 extracted from

  • Huang, N.; Agrawal, V.; Giacomini, K.M.; Miller, W.L.
    Genetics of P450 oxidoreductase: sequence variation in 842 individuals of four ethnicities and activities of 15 missense mutations (2008), Proc. Natl. Acad. Sci. USA, 105, 1733-1738.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of missense variant proteins lacking 27 N-terminal residues in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
A462T vmax/Km for ferricytochrome c is 85% of the wild-type value. Vmax/Km for NADPH is 69% of the wild-type value Homo sapiens
A485T vmax/Km for ferricytochrome c is 36% of the wild-type value. Vmax/Km for NADPH is 51% of the wild-type value Homo sapiens
A503V vmax/Km for ferricytochrome c is 67% of the wild-type value. Vmax/Km for NADPH is 56% of the wild-type value Homo sapiens
D211L vmax/Km for ferricytochrome c is 27% of the wild-type value. Vmax/Km for NADPH is 59% of the wild-type value Homo sapiens
delE53 vmax/Km for ferricytochrome c is 57% of the wild-type value. Vmax/Km for NADPH is 93% of the wild-type value Homo sapiens
E300K vmax/Km for ferricytochrome c is 93% of the wild-type value. Vmax/Km for NADPH is 104% of the wild-type value Homo sapiens
G213E vmax/Km for ferricytochrome c is 111% of the wild-type value. Vmax/Km for NADPH is 105% of the wild-type value Homo sapiens
P284L vmax/Km for ferricytochrome c is 104% of the wild-type value. Vmax/Km for NADPH is 153% of the wild-type value Homo sapiens
P284T vmax/Km for ferricytochrome c is 16% of the wild-type value. Vmax/Km for NADPH is 32% of the wild-type value Homo sapiens
P452L vmax/Km for ferricytochrome c is 16% of the wild-type value. Vmax/Km for NADPH is 12% of the wild-type value Homo sapiens
P55L vmax/Km for ferricytochrome c is 67% of the wild-type value. Vmax/Km for NADPH is 123% of the wild-type value Homo sapiens
R406H vmax/Km for ferricytochrome c is 62% of the wild-type value. Vmax/Km for NADPH is 78% of the wild-type value Homo sapiens
R600W vmax/Km for ferricytochrome c is 18% of the wild-type value. Vmax/Km for NADPH is 7% of the wild-type value Homo sapiens
V472M vmax/Km for ferricytochrome c is 23% of the wild-type value. Vmax/Km for NADPH is 24% of the wild-type value Homo sapiens
Y607C Vmax/Km for NADPH is 20% of the wild-type value Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025
-
NADPH mutant enzyme Y607C Homo sapiens
0.066
-
NADPH mutant enzyme P284T Homo sapiens
0.084
-
NADPH mutant enzyme R600W Homo sapiens
0.125
-
NADPH mutant enzyme P452L Homo sapiens
0.144
-
NADPH wild-type enzyme Homo sapiens
0.144
-
NADPH mutant enzyme A485T Homo sapiens
0.146
-
NADPH mutant enzyme P55L Homo sapiens
0.151
-
NADPH mutant enzyme delE53 Homo sapiens
0.153
-
NADPH mutant enzyme D211L Homo sapiens
0.155
-
NADPH mutant enzyme A503V Homo sapiens
0.157
-
NADPH mutant enzyme P284L Homo sapiens
0.169
-
NADPH mutant enzyme V472M Homo sapiens
0.177
-
NADPH mutant enzyme R406H Homo sapiens
0.196
-
NADPH mutant enzyme G213E Homo sapiens
0.222
-
NADPH mutant enzyme E300K Homo sapiens
0.276
-
NADPH mutant enzyme A462T Homo sapiens
0.39
-
ferricytochrome c mutant enzyme R600W Homo sapiens
0.92
-
ferricytochrome c mutant enzyme P452L Homo sapiens
0.99
-
ferricytochrome c mutant enzyme A503V Homo sapiens
1.15
-
ferricytochrome c mutant enzyme P284T Homo sapiens
1.19
-
ferricytochrome c wild-type enzyme Homo sapiens
1.37
-
ferricytochrome c mutant enzyme V472M Homo sapiens
1.61
-
ferricytochrome c mutant enzyme G213E Homo sapiens
1.84
-
ferricytochrome c mutant enzyme A485T Homo sapiens
1.86
-
ferricytochrome c mutant enzyme E300K Homo sapiens
2.07
-
ferricytochrome c mutant enzyme R406H Homo sapiens
2.09
-
ferricytochrome c mutant enzyme A462T Homo sapiens
2.16
-
ferricytochrome c mutant enzyme delE53 Homo sapiens
2.25
-
ferricytochrome c mutant enzyme P284L Homo sapiens
2.31
-
ferricytochrome c mutant enzyme P55L Homo sapiens
2.5 5 ferricytochrome c mutant enzyme D211L Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ferricytochrome c + NADPH
-
Homo sapiens 2 ferrocytochrome c + NADP+ + H+
-
?

Synonyms

Synonyms Comment Organism
P450 oxidoreductase
-
Homo sapiens
POR
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Homo sapiens