Cloned (Comment) | Organism |
---|---|
the complete CPR cDNA is expressed as 6*His-tagged fusion protein in both membrane and cytosolic fractions in Escherichia coli. Both fractions contain NADPH-cytochrome c reducing activity. The membrane-bound form containing N-terminal membrane anchor is subjected to purification | Anopheles minimus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
77300 | - |
x * 77300, calculated from sequence | Anopheles minimus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Anopheles minimus | A4ZIT2 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme, membrane-bound | Anopheles minimus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ferricytochrome c + NADPH | - |
Anopheles minimus | 2 ferrocytochrome c + NADP+ + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 77300, calculated from sequence | Anopheles minimus |
Synonyms | Comment | Organism |
---|---|---|
NADPH-cytochrome P450 reductase | - |
Anopheles minimus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Anopheles minimus | |
FMN | - |
Anopheles minimus |