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Literature summary for 1.6.2.4 extracted from
- Detergent-solubilized NADPH-cytochrome c(P-450) reductase from the higher plant, Catharanthus roseus. Purification and characterization (1979), J. Biol. Chem., 254, 2419-2427.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | increasing ionic strength, increase in activity | Catharanthus roseus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| cetyltrimethyl ammonium bromide | - |
Catharanthus roseus |  |
| NADP+ | competitive inhibition | Catharanthus roseus | |
| p-chloromercuribenzoate | - |
Catharanthus roseus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0057 | - |
NADPH | - |
Catharanthus roseus | |
| 0.0078 | - |
ferricytochrome c | - |
Catharanthus roseus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| additional information | - |
Catharanthus roseus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | stimulation by increasing ionic strength | Catharanthus roseus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Catharanthus roseus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Catharanthus roseus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purified 120fold by a combination of ion exchange chromatography and gel filtration and 745fold by affinity chromatography on 2',5'-ADP-Sepharose 4B | Catharanthus roseus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seedling | - |
Catharanthus roseus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.245 | - |
purification by ion exchange chromatography and gel filtration, electron acceptor: dichlorophenolindophenol | Catharanthus roseus |
| 1.12 | - |
purification by ion exchange chromatography and gel filtration, electron acceptor: ferricyanide | Catharanthus roseus |
| 1.429 | - |
purification by ion exchange chromatography and gel filtration | Catharanthus roseus |
| 17.14 | - |
purification by affinity chromatography on 2',5'-ADP-Sepharose 4B | Catharanthus roseus |
| 22 | - |
in the presence of 0.005 mM FMN | Catharanthus roseus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 ferricytochrome c + NADPH | additional electron acceptor: ferricyanide | Catharanthus roseus | 2 ferrocytochrome c + NADP+ + H+ | - |
? | |
| 2 ferricytochrome c + NADPH | additional electron acceptor: cytochrome P450 | Catharanthus roseus | 2 ferrocytochrome c + NADP+ + H+ | - |
? | |
| 2 ferricytochrome c + NADPH | additional electron acceptor: 2,6-dichlorophenolindophenol | Catharanthus roseus | 2 ferrocytochrome c + NADP+ + H+ | - |
? | |
| NADPH + H+ + ferricytochrome c | - |
Catharanthus roseus | NADP+ + ferrocytochrome c | - |
r | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Catharanthus roseus | |
| FMN | - |
Catharanthus roseus | |
| NADPH | - |
Catharanthus roseus | |
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