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Literature summary for 1.6.2.2 extracted from
- NADH cytochrome b5 reductase and cytochrome b5 catalyze the microsomal reduction of xenobiotic hydroxylamines and amidoximes in humans (2004), J. Pharmacol. Exp. Ther., 311, 1171-1178.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli as His-tag fusion protein | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.36 | - |
sulfamethoxazole hydroxylamine | pH 7.4 | Homo sapiens |  |
| 0.36 | - |
dapsone hydroxylamine | pH 7.4 | Homo sapiens | |
| 0.63 | - |
benzamidoxime | pH 7.4 | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Homo sapiens | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36000 | - |
SDS-PAGE, 32000 Da protein + 4000 Da His-tag | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 ferricytochrome b5 + NADH | Homo sapiens | involved in the synthesis of fatty acids and cholesterol, and in the oxidation of xenobiotics | 2 ferrocytochrome b5 + NAD+ + H+ | - |
? | |
| benzamidoxime + NADH | Homo sapiens | in the presence of cytochrome b5 | ? | - |
? | |
| dapsone hydroxylamine + NADH | Homo sapiens | in the presence of cytochrome b5 | ? | - |
? | |
| sulfamethoxazole hydroxylamine + NADH | Homo sapiens | in the presence of cytochrome b5 | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Homo sapiens | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, phosphate buffered saline, pH 7.4, protein concentration 2-3 mg/ml, stable for 3 weeks | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 ferricytochrome b5 + NADH | - |
Homo sapiens | 2 ferrocytochrome b5 + NAD+ + H+ | - |
? | |
| 2 ferricytochrome b5 + NADH | involved in the synthesis of fatty acids and cholesterol, and in the oxidation of xenobiotics | Homo sapiens | 2 ferrocytochrome b5 + NAD+ + H+ | - |
? | |
| benzamidoxime + NADH | in the presence of cytochrome b5 | Homo sapiens | ? | - |
? | |
| dapsone hydroxylamine + NADH | in the presence of cytochrome b5 | Homo sapiens | ? | - |
? | |
| sulfamethoxazole hydroxylamine + NADH | in the presence of cytochrome b5 | Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| diaphorase I | - |
Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Homo sapiens | |
| NADH | - |
Homo sapiens | |
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Release 2023.1 (January 2023)
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