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Literature summary for 1.6.2.2 extracted from

  • Yang, M.X.; Cederbaum, A.I.
    Fractionation of liver microsomes with polyethylene glycol and purification of NADH-cytochrome b5 oxidoreductase and cytochrome b5 (1994), Arch. Biochem. Biophys., 315, 438-444.
    View publication on PubMed
Search for more literature for 1.6.2.2

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33100
-
 1 * 33100, SDS-PAGE Rattus norvegicus
33100
-
 1 * 33100, SDS-PAGE Oryctolagus cuniculus

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
polyethylene glycol precipitation, ADP-agarose Oryctolagus cuniculus
polyethylene glycol precipitation, DEAE-cellulose, hydroxylapatite, ADP-agarose Rattus norvegicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1575
-
-
 Oryctolagus cuniculus
1679
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Fe3+-ammonium sulfate + NADH strongly elevated by the addition of cytochrome b5 Rattus norvegicus Fe2+-ammonium sulfate + NAD+
-
 ?
Fe3+-ATP + NADH ferric-EDTA is not reduced Rattus norvegicus Fe2+-ATP + NAD+
-
 ?
Fe3+-histidine + NADH strongly elevated by the addition of cytochrome b5 Rattus norvegicus Fe2+-histidine + NAD+
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 33100, SDS-PAGE Rattus norvegicus
monomer 1 * 33100, SDS-PAGE Oryctolagus cuniculus