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Literature summary for 1.6.2.2 extracted from

  • Kubota, S.; Yoshida, Y.; Kumaoka, H.
    Studies on the microsomal electron-transport system of anaerobically grown yeast. IV. Purification and characterization of NADH-cytochrome b5 reductase (1977), J. Biochem., 81, 187-195.
    View publication on PubMed

General Stability

General Stability Organism
unstable, 40% loss of activity at 0°C overnight, 1 mg/ml gelatin stabilizes, FAD protects against inactivation Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
Saccharomyces cerevisiae
2,2'-dipyridyl
-
Saccharomyces cerevisiae
Hg2+
-
Saccharomyces cerevisiae
K+ high ionic strength
-
Saccharomyces cerevisiae
NAD+ competitive Saccharomyces cerevisiae
p-chloromercuribenzoate
-
Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0008
-
ferricytochrome b5
-
Saccharomyces cerevisiae
0.0008
-
ferricytochrome b5 yeast cytochrome b5 Saccharomyces cerevisiae
0.0015
-
ferricytochrome b5 calf cytochrome b5 Saccharomyces cerevisiae
0.0063
-
NADH
-
Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
Saccharomyces cerevisiae 5783
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
gel filtration Saccharomyces cerevisiae
28000
-
1 * 28000, SDS-PAGE Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
grown anaerobically
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
69.8
-
-
Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ferricytochrome b5 + NADH specific for NADH as electron donor, artificial acceptors: ferricyanide, 2,6-dichlorphenolindophenol Saccharomyces cerevisiae 2 ferrocytochrome b5 + NAD+ + H+
-
?
NADH + ferricytochrome b5
-
Saccharomyces cerevisiae NAD+ + H+ + ferrocytochrome b5
-
r

Subunits

Subunits Comment Organism
monomer 1 * 28000, SDS-PAGE Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6
-
steady decrease above Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
NADH
-
Saccharomyces cerevisiae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.23
-
NAD+
-
Saccharomyces cerevisiae