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Literature summary for 1.6.2.2 extracted from

  • Lostanlen, D.; De Barro, A.V.; Leroux, A.; Kaplan, J.C.
    Soluble NADH-cytochrome b5 reductase from rabbit liver cytosol: partial purification and characterization (1978), Biochim. Biophys. Acta, 526, 42-51.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
p-hydroxymercuribenzoate 0.1 mM, complete inhibition Oryctolagus cuniculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.008
-
methemoglobin-ferrocyanide complex
-
Oryctolagus cuniculus
0.01
-
ferricyanide
-
Oryctolagus cuniculus
0.015
-
cytochrome b5
-
Oryctolagus cuniculus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Oryctolagus cuniculus 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
liver, cytosol, gel filtration, sucrose density gradient centrifugation Oryctolagus cuniculus
30000
-
enzyme from liver cytosol, gel filtration Oryctolagus cuniculus

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.69
-
methemoglobin-ferrocyanide reductases assay Oryctolagus cuniculus

Storage Stability

Storage Stability Organism
-20°C, several months, no loss of activity Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ferricytochrome b5 + NADH specific for NADH as electron donor, artificial acceptors: ferricyanide, 2,6-dichlorphenolindophenol Oryctolagus cuniculus 2 ferrocytochrome b5 + NAD+ + H+
-
?
2 ferricytochrome b5 + NADH additional acceptor: methemoglobin-ferrocyanide complex Oryctolagus cuniculus 2 ferrocytochrome b5 + NAD+ + H+
-
?
methemoglobin-ferrocyanide complex + NADH
-
Oryctolagus cuniculus reduced methemoglobin-ferrocyanide complex + NAD+
-
?
NADH + ferricyanide
-
Oryctolagus cuniculus NAD+ + H+ + ferrocyanide
-
r
NADH + ferricytochrome b5
-
Oryctolagus cuniculus NAD+ + H+ + ferrocytochrome b5
-
r

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
Tris-phosphate buffer Oryctolagus cuniculus
7
-
triethanolamine buffer Oryctolagus cuniculus

Cofactor

Cofactor Comment Organism Structure
NADH
-
Oryctolagus cuniculus