Cloned (Comment) | Organism |
---|---|
domains dIII and dI expressed in Escherichia coli, recombinant protein lacks the membrane spanning dII domain | Entamoeba histolytica |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Entamoeba histolytica | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
dIII fragement, SDS-PAGE | Entamoeba histolytica |
40000 | - |
dI fragement, SDS-PAGE | Entamoeba histolytica |
155000 | - |
gel filtration of a mixture of the two domains | Entamoeba histolytica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADH + NADP+ | Entamoeba histolytica | links hydride transfer between NAD(H) and NADP(H) to the translocation of protons across membrane, important as source of NADPH for biosynthesis and glutathione reduction | NAD+ + NADPH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Entamoeba histolytica | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant proteins | Entamoeba histolytica |
Storage Stability | Organism |
---|---|
-80°C, 25% glycerol | Entamoeba histolytica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADH + NADP+ | - |
Entamoeba histolytica | NAD+ + NADPH | - |
r | |
NADH + NADP+ | links hydride transfer between NAD(H) and NADP(H) to the translocation of protons across membrane, important as source of NADPH for biosynthesis and glutathione reduction | Entamoeba histolytica | NAD+ + NADPH | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * (40000 + 28000), SDS-PAGE, domains dIII and dI form active dimers | Entamoeba histolytica |
Synonyms | Comment | Organism |
---|---|---|
proton-translocating transhydrogenase | - |
Entamoeba histolytica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD(P)+ | - |
Entamoeba histolytica | |
NAD(P)H | - |
Entamoeba histolytica |