Cloned (Comment) | Organism |
---|---|
expression of domains I and III in Escherichia coli | Rhodospirillum rubrum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodospirillum rubrum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant domains I and III | Rhodospirillum rubrum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADH + oxidized 3-acetylpyridine adenine dinucleotide | - |
Rhodospirillum rubrum | NAD+ + reduced 3-acetylpyridine adenine dinucleotide | - |
r | |
NADPH + oxidized 3-acetylpyridine adenine dinucleotide | catalyzed by a mixture of purified recombinant domains I and III | Rhodospirillum rubrum | NADP+ + reduced 3-acetylpyridine adenine dinucleotide | - |
r |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
oxidized 3-acetylpyridine adenine dinucleotide | kcat for recombinant domain III in the presence of saturating amounts of domain I, reduction of acetylpyridine adenine dinucleotide by NADPH | Rhodospirillum rubrum | |
1.44 | - |
oxidized 3-acetylpyridine adenine dinucleotide | kcat for recombinant domain I in the presence of saturating amounts of domain III, reduction of acetylpyridine adenine dinucleotide by NADPH | Rhodospirillum rubrum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Rhodospirillum rubrum | |
NADH | - |
Rhodospirillum rubrum | |
NADP+ | - |
Rhodospirillum rubrum | |
NADPH | - |
Rhodospirillum rubrum |